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INVITRO ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF Carica papaya AND Azadirachta indica LEAF AND STEM BARK EXTRACTS ON SELECTED CLINICAL ISOLATES

OWOLABI, Adebayo Oladipo and ABAH, K. A. and Oranusi, S. U. (2017) INVITRO ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF Carica papaya AND Azadirachta indica LEAF AND STEM BARK EXTRACTS ON SELECTED CLINICAL ISOLATES. Journal of Industrial Research and Technology, 6 (1). pp. 209-220.

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Abstract

The search for alternative sources of antibiotic is a global challenge due to the increase in the emergence of resistant strams. Plants have been in use in traditional medicine before the era of chemotherapeutics and about 80% of the global population still uses them . . Azadirachta indica (neem) and Carica papaya are trees that have been found to possess antibacterial, antifungal, antiinflammatory, anti-tumour properties and also used as a pest icide. In this work, antibacterial, phytochemical and antioxidant potentials of ethanolic and aqueous extracts of Carica papaya and Azadirachta indica leaf and stem bark was determined using antimicrobial sensitivity assay, minimum inhibitory concentration, minimum lethal concentration, Ferric reducing antioxidant power assay and Total antioxidant activity of extracts as indices. The test organisms were Escherichia coli, Salmonella typhimurium, Bacillus subtilis and Staphylococcus au reus. Azadirachta indica leaf water extract and Azadirachta indica stem bark ethanol extract showed a clear zone of inhibition ranging from 10±0mm to 15.5±0.71mm and 10±0mm to 15.5±2.12mm respectively against all four test isolates, while others extracts had clear zones of inhibition against at least three test isolates with inhibition zones ranging from 10.5±0. 71mm to 15±1.41mm. Ethanolic extract of Carica papaya leaf was active against Bacillus subtilis alone (11.5±0.71mm).Some combined extracts expressed activity against all four isolate, while the highest individual extract inhibition zone was 15.5±2.12mm, combined extract was 18.5±0. 71mm against Salmonella typhimurium. All extracts had antioxidant activity and some of the phytochemicals present in the extracts include saponins, flavonoids, tannins, anthocyanin, betacyanin, quinones, cardiac glycoside, terpenoids, and phenols. However further research is still needed to identify the active phytochemica/s and their concentrations in the extracts

Item Type: Article
Uncontrolled Keywords: Azadirachta indica, Carica papaya, Antimicrobial, phytochemical, antioxidants
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mrs Hannah Akinwumi
Date Deposited: 25 Jan 2018 12:47
Last Modified: 25 Jan 2018 12:47
URI: http://eprints.covenantuniversity.edu.ng/id/eprint/10114

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