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Efficiency of Histidine Rich Protein II-Based Rapid Diagnostic Tests for Monitoring Malaria Transmission Intensities in an Endemic Area

Dokunmu, Titilope M. and Olasehinde, G. I and Oladejo, David Oladoke and Olanrewaju, Oladeji and Akinbobola, Abisola and Adjekukor, Cynthia Ufuoma and Yakubu, Omolara Faith and Adebayo, H. A. (2018) Efficiency of Histidine Rich Protein II-Based Rapid Diagnostic Tests for Monitoring Malaria Transmission Intensities in an Endemic Area. In: 2nd International Conference on Applied Sciences (ICAS-2), 2018.

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Abstract

In recent years there has been a global decrease in the prevalence of malaria due to scaling up of control measures, hence global control efforts now target elimination and eradication of the disease. However, a major problem associated with elimination is asymptomatic reservoir of infection especially in endemic areas. This study aims to determine the efficiency of histidine rich protein II (HRP-2) based rapid diagnostic tests (RDT) for monitoring transmission intensities in an endemic community in Nigeria during the pre-elimination stage. Plasmodium falciparum asymptomatic malaria infection in healthy individuals and symptomatic cases were detected using HRP-2. RDT negative tests were re-checked by microscopy and by primer specific PCR amplification of merozoite surface protein 2 (msp-2) for asexual parasites and Pfs25 gene for gametocytes in selected samples to detect low level parasitemia undetectable by microscopy. The mean age of the study population (n=280) was 6.12 years [95% CI 5.16 – 7.08, range 0.5 – 55], parasite prevalence was 44.6% and 36.3% by microscopy and RDT respectively (p =0.056). The parasite prevalence of 61.5% in children aged >2 – 10 years was significantly higher than 3.7% rate in adults >18years (p < 0.0001, χ2 = 60.45). RDT detected additional 29.6% asymptomatic cases but a lower specificity of 68.8% in symptomatic carriers. In 15 selected RDT positive samples, only 6 were positive by PCR and no gametocyte was detected. The results indicate that HRP-2 RDTs are a vital tool for understanding transmission dynamics and detecting immune-suppressed, recent and asymptomatic infections, thus crucial to tackle low level transmission and eliminating malaria in endemic areas.

Item Type: Conference or Workshop Item (Paper)
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
R Medicine > RA Public aspects of medicine
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mrs Patricia Nwokealisi
Date Deposited: 20 Jun 2018 10:46
Last Modified: 20 Jun 2018 10:46
URI: http://eprints.covenantuniversity.edu.ng/id/eprint/10976

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