Bioactive Screening and In Vitro Antioxidant Assessment of Nauclea latifolia Leaf Decoction

Iheagwam, Franklyn N and Nsedu, Emmanuel Israel and Kayode, Kazeem Oyindamola and Emiloju, Opeyemi C. and Ogunlana, Olubanke Olujoke and Chinedu, S. N. (2018) Bioactive Screening and In Vitro Antioxidant Assessment of Nauclea latifolia Leaf Decoction. In: 2nd International Conference on Applied Sciences (ICAS-2), 2018.

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Abstract

The phytochemical constituents and antioxidant properties of Nauclea latifolia leaf decoction were investigated. Dried leaves were extracted in ethanol. Qualitative and quantitative phytochemical analysis was determined spectrometrically. The antioxidant activities were examined in vitro using 2,2-diphenyl-1-picrylhydrazyl radical, total antioxidant capacity and ferric reducing antioxidant power assays. Phytochemical screening confirmed the presence of flavonoids, alkaloids, anthocyanins, betacyanins, phenols, saponins, terpenoids, cardiac glycosides and quinones. The total lycopene, β-carotene, phenolics, flavonoid and alkaloid content were found to be 0.038 ± 0.01 mg CAE/g, 0.120 ± 0.04 mg CAE/g, 58.08 ± 0.58 mg GAE/g, 10.75 ± 0.17 mg RE/g and 0.32 ± 0.08% respectively. N. latifolia ethanol leaf extract demonstrated effective antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl with an IC50 of 2.58 ± 0.08 mg/mL compared to 0.86 ± 0.02 mg/mL and < 0.01 ± 0.01 mg/mL for butylated hydroxytoluene and ascorbic acid respectively. Total antioxidant capacity and ferric reducing antioxidant power of the extract were 73.81 ± 2.27 and 1314.45 ± 197.64 mg AAE/g respectively. Excellent positive correlations between the phenolic content and antioxidant activities of the extract were observed. The leaf of N. latifolia is of therapeutic value and may be exploited for its rich antioxidant components

Item Type:	Conference or Workshop Item (Paper)
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	Mrs Patricia Nwokealisi
Date Deposited:	25 Jun 2018 10:02
Last Modified:	25 Jun 2018 10:02
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/11029

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