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Solenostemon monostachyus Modulates Inducible Nitric Oxide Synthase and mRNA Expression in Hemolytic-Induced Rats

Afolabi , Israel Sunmola and Okafor, Adaobi and Osikoya, I.O and Akinbo, Bolaji David and Rotimi, S. O and Adebiyi, E. F. (2017) Solenostemon monostachyus Modulates Inducible Nitric Oxide Synthase and mRNA Expression in Hemolytic-Induced Rats. Journal of Biological Sciences, 17 (8). pp. 353-261. ISSN 1727-3048

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Background and Objective: The 2-Butoxyethanol (2BE) rat model of human hemolytic disorders has been used widely to evaluate hemolysis and thromboembolic manifestations of different organs associated with sickle cell disease and $-thalassemia, however, studies on nitric oxide metabolism are far more scarce. This study investigated the effects of Solenostemon monostachyus leaf extract supplementation on 2BE-induced changes of inducible nitric oxide synthase (iNOS) levels and mRNA expression in female rats. Materials and Methods: Young female rats were divided into seven groups (Group A-G). All groups, except for group A, were exposed to 2BE. The treatments assignments were as follows, group C: Caffeic acid, group D: Ciklavit (herbal formulation of Cajanus cajan (L.) Mill sp.), group E-G: S. monostachyus (150, 200 and 250 mg kgG1 b.wt.), respectively. Liver and plasma iNOS levels, as well as mRNA expression of liver arginase-1 and iNOS, were assessed. Histopathological evaluation of the liver was also performed. The results were statistically analyzed for significant effects at p<0.05 using one-way analysis of variance (ANOVA). Results: Liver and plasma iNOS were significantly reduced (p<0.05) in group G, whereas liver iNOS was significantly increased (p<0.05) in group D-F compared with group B, iNOS mRNA expression was reduced, while arginase-1 mRNA expression was increased in group E-G compared with group B. Conclusion: Administration of S. monostachyus (250 mg kgG1 b.wt.) in the 2BE hemolytic rat model modulated plasma and liver iNOS levels and iNOS mRNA expression.

Item Type: Article
Uncontrolled Keywords: Solenostemon monostachyus , hemolysis, thrombosis, inducible nitric oxide synthase, arginase
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mrs Patricia Nwokealisi
Date Deposited: 11 Jul 2018 11:40
Last Modified: 11 Jul 2018 11:40

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