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Antiplasmodial Activity shown by Secondary Metabolites Extracted from the Seeds ofPentaclethramacrophyllaBenth

Olasehinde, G. I and Mordi, Raphael C. and Eluagwule, Arnold N. and Jonathan, Hassana and Ayo-Ajayi, J. I. and Okedere, Adebola P. and Uchenna, Daniel .O. and Oluseye, Oreoluwa R. and Diji-Geske, Ruth I. and Onile-ere, Olabode and Azuh, Dominic E. (2018) Antiplasmodial Activity shown by Secondary Metabolites Extracted from the Seeds ofPentaclethramacrophyllaBenth. In: IOP Conference Series: Earth and Environmental Science, 2018.

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Oil extracts from the African oil bean seed (PentaclethramacrophyllaBenth.) was analyzed for its phytochemical and mineral content and proximate, physicochemical and antimicrobial analyses were also performed. Phytochemical analysis showed the presence of tannins, saponins, quinones, terpenoids, phenols and coumarins in the oil sample. Mineral determination of the cotyledon showed the presence of iron (Fe) (with the highest concentration), Cu, Zn, Mn, Cr, Pb and Cd; while proximate analysis gave the following result: moisture (14.2%), ash content (1.5%), crude fibre (4.9%), crude proteins (12.8%), oil contents (4.9%), and carbohydrate (61.8%). GC-MS analysis of the partitioned petroleum ether and chloroform fractions of the oil revealed the presence of 9-Octadecenoic acid, 9,12- Octadecadienoic acid and their methyl esters,cis-9-Hexadecenal among the many components of the oil extract. Physicochemical analysis of the oil indicateda saponification value (148.67 mg KOH/g), peroxide value(8.0 meq/g), iodine value (10.41 mg iodine/g) and free fatty acid (8.98 mg KOH/g). The need for the development of new drugs for malaria led to our study of the antiplasmodial activity of the oil from the seeds of Pentaclethramacrophylla. Toxicological studies were carried out to determine the LD50with chloroquinediphosphate as positive control and normal saline as negative control. Using the Peter’s 4 day suppressive test a parasite inhibition rate of 47.72% (25 mg/kg), 63.63% (50 mg/kg) and 61.36% (100 mg/kg) on day 4 after treatment was recorded. A 95.45% chemo-suppression was observed for animals treated with 10 mg/kg chloroquine. This resultis an indication that the extract had appreciable signs of chemosuppression.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Pentaclethramacrophylla, phytochemical analysis, antimicrobial analysis, GC-MS, Plasmodium, secondary metabolites, chemo-suppression, malaria
Subjects: H Social Sciences > HB Economic Theory
Q Science > QD Chemistry
Divisions: Faculty of Engineering, Science and Mathematics > School of Chemistry
Faculty of Law, Arts and Social Sciences > School of Social Sciences
Depositing User: Mrs Patricia Nwokealisi
Date Deposited: 19 Jul 2018 08:18
Last Modified: 19 Jul 2018 08:18

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