Plant Waste Hydrolysis by Extracellular Enzymes of Aspergillus niger and Penicillium chrysogenum: Effect of Ammonia Pretreatment

Chinedu, S. N. and Yah, S. C. and Nwinyi, Obinna and Okochi, V. I. and Okafor, U. A. and Onyegeme-Okerenta, B. M. (2008) Plant Waste Hydrolysis by Extracellular Enzymes of Aspergillus niger and Penicillium chrysogenum: Effect of Ammonia Pretreatment. Nigerian Journal of Biochemistry and Molecular Biology. ISSN 0189-4757

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Abstract

Aspergillus niger (ANL301) and Penicillium chrysogenum (PCL 501) cultured in basal media with cellulose as sole carbon source yielded extracellular enzymes which partially hydrolyzed sawdust and sugarcane pulp into simple sugars. Pre-treatment of sawdust by ammonium hydroxide steeping increased the yield of simple sugars. The reduc-ing sugars released from the pretreated sawdust by the crude enzymes of A. niger (ANL301) and P. chrysogenum (PCL 501) were 3.58% and 7.02% of the total hydrolysable sugars respectively. This is in contrast to the 0.92% and 1.02% of the total hydrolysable sugars released respectively by the enzymes of A. niger (ANL301) and P. chry-sogenum (PCL 501) from the non-pretreated sawdust. Enzymatic hydrolysis of sugarcane pulp by the crude enzymes was not significantly affected by ammonia pre-treatment. Reducing sugars released from non-pretreated sugarcane pulp by the crude enzymes of A. niger (ANL301) and P. chrysogenum (PCL 501) were respectively 4.17% and 5.08% of the total hydrolysable sugars.

Item Type:	Article
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	Mr Adewole Adewumi
Date Deposited:	09 Apr 2011 22:05
Last Modified:	13 Dec 2011 21:13
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/113

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