Stevioside modulates oxidative damage in the liver and kidney of high fat/low streptozocin diabetic rats

Rotimi, S. O and Rotimi, Oluwakemi A and Adelani, Isaacson and Onuzulu, Chinonye and Obi, Patience O. and Okungbaye, Rotimi (2018) Stevioside modulates oxidative damage in the liver and kidney of high fat/low streptozocin diabetic rats. Heliyon.

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Abstract

This study investigated the potential of stevioside to prevent oxidative DNA damage in the liver and kidney of type 2 diabetes mellitus (T2DM) using high fat-low streptozocin rat model. Rats were treated daily with 12.5, 25 and 50 mg/ kg stevioside orally for 21 days. Levels of biomarkers of T2DM, lipid profile and oxidative stress were assayed spectrophotometrically. The DNA ladder assay method was used to assess DNA fragmentation in the liver and kidney while computational analysis was used to predict the mechanisms of antidiabetic properties of stevioside. Stevioside significantly (p < 0.05) decreased the levels of plasma glucose, insulin, dipeptidyl peptidase IV and activities of kidney angiotensin converting enzyme. Stevioside significantly reduced oxidative stress by decreasing the levels of lipid peroxidation and nitric oxide in the liver and kidney; thereby, reducing the extent of DNA fragmentation in the liver and kidney of the diabetic rats. The in silico analysis showed that the ability of stevioside to exert these effects is linked to its inhibition of beta-adrenergic receptor kinase and G-protein-coupled receptor kinase. The results of this study suggest that the prevention of DNA fragmentation may be an additional benefit of the use of stevioside in the management of T2DM.

Item Type:	Article
Uncontrolled Keywords:	Nutrition, Metabolism
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	Mrs Hannah Akinwumi
Date Deposited:	24 Sep 2018 11:52
Last Modified:	24 Sep 2018 11:52
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/11890

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