Obafemi, Yemisi Dorcas and Ajayi, A. A. and Taiwo, O. S. and Olorunsola, S. J. and Isibor, Patrick Omoregie (2019) Isolation of Polygalacturonase-Producing Bacterial Strain from Tomatoes (Lycopersicon esculentum Mill.). International Journal of Microbiology Research. ISSN 0975-5276 & E- 0975-9174
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Abstract
Background. Polygalacturonase (EC 3.2.1.15) enzyme aids in microbial spoilage of fruits and vegetables. It is very important to find economical ways to producing the enzyme so as to achieve maximum yield in industries due to its use at different areas of production process. Methods. Isolation of polygalacturonase-producing bacterial strain from tomatoes (Lycopersicon esculentum Mill.) was studied. Polygalacturonase-producing bacterial strains were isolated and screened from tomatoes stored at normal laboratory temperature (25 ± 2°C). +ey were identified based on their morphological, biochemical, and molecular characteristics. +e enzyme produced was partially purified by the ammonium sulphate precipitation method. Molecular weights and optimum conditions for best enzyme activity were obtained by SDS PAGE technique. Results. Five bacterial isolates resulted after screening. Bacterial strain code B5 showed highest polygalacturonase activity. Optimum conditions for polygalacturonase PEC B5 were maintained at pH 4.5; temperature 35°C; substrate concentration 0.3 mg/ml, and best activity at less than 5 min of heating. +e enzyme PEC B5 was found to weigh 65 kDa and 50 kDa for crude and partially purified aliquots, respectively. +e result of 16S rRNA gene sequencing revealed bacterial strain code B5 as Enterobacter tabaci NR146667 having 79% similarity with the NCBI GenBank. Conclusion. Microorganisms should be developed for large-scale production of enzymes in developing countries.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | Mrs Patricia Nwokealisi |
| Date Deposited: | 25 Nov 2019 11:21 |
| Last Modified: | 25 Nov 2019 11:21 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/12984 |
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