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Multi-antibiotics Resistant Relatedness of bla-gene Encoded Enteric Bacteria harbouring High Molecular R-plasmids.

Akinduti, P. A. and OLUWADUN, AFOLABI and Osiyemi, J. and Ejilude, Oluwaseun and Isibor, Patrick Omoregie (2019) Multi-antibiotics Resistant Relatedness of bla-gene Encoded Enteric Bacteria harbouring High Molecular R-plasmids. In: 8th International Biotechnology Conference, Exhibition and Workshop, 2019, Online.

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Increase prevalence of multi-resistant enteric bacteria isolates encoded with high mobile R-plasmid causing enteric infections was examined among the community residents in Abeokuta, Nigeria. Random cluster sampling of 251 fecal samples of community residents were cultured for enteric bacteria and biotyped. Disc diffusion and Micro-broth dilution assay were used to determine antibiotic susceptibility while R-plasmid was profiled with photo-gel documentation. Antibiotic resistance relatedness was detected using DendroUPGMA construction utility software. Of all isolates obtained, 31.3% were Escherichia coli Klebsiella oxytoca (19.5%), Pseudomonas aeruginosa (15.3%) and Shigella specie (2.0%). Significant high rate of 62.6% showed resistant to Cefuroxime, 61.6% to Ampicillin and Augmentin (54.2%) while 44.7%, 38.9% and 33.9% resist Cotrimoxazole, Ciprofloxacin and Tetracycline respectively at MIC >16 μg/ml (p= 0.004). Only 54.1% harboured high molecular weight R-plasmid (>11.0kbp) and 2.7% having <5kbp R-plasmid weight. Two distinct clusters revealed significant multi-antibiotic resistant relatedness. Cluster A enteric isolates harboured similar R-plasmid of only one bands with high molecular weight more than 11kbp while Cluster B divided into subgroup a and subgroup b comprising different enteric species having similar high molecular weights with high antibiotic resistant expressing more than two plasmid bands showing computed cophenetic correlation of 0.94. Cluster analysis reveal a related high level multi-antibiotics resistant enteric bacteria strains among the community residents suggesting a continuous dissemination and imminent outbreak of resistant enteric pathotypes with resultant epidemic proportion.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Antibiotics, Resistant, R-Plasmid, Bacilli
Subjects: Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mrs Patricia Nwokealisi
Date Deposited: 28 Nov 2019 14:56
Last Modified: 28 Nov 2019 14:56

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