OLADEJO, DAVID OLADOKE and Covenant University, Theses (2018) ASSESSMENT OF MOLECULAR MARKERS ASSOCIATED WITH ARTEMISININ RESISTANCE IN Plasmodium falciparum ISOLATES FROM OTA, SOUTHWEST NIGERIA. Masters thesis, COVENANT UNIVERSITY.
![[img]](http://eprints.covenantuniversity.edu.ng/style/images/fileicons/other.png) |
PDF
Download (385Kb) |
Abstract
Antimalarial drug resistance means delayed malaria parasites clearance from the peripheral blood of a patient treated with the drug. Artemisinin resistance (AR) has developed in Southeast Asia owing to mutations in parasite genes. From historical spread of drug resistance across Asia to Africa, the spread of resistance to artemisinin combination therapy across Asia to Africa is imminent. This study evaluates molecular markers associated with artemisinin derivatives and partner drugs commonly used in Africa and provides data on the Plasmodium falciparum multidrug resistant 1 (Pfmdr1) and the Kelch propeller (K13) gene mutation profile of few parasite isolates from Ota, Southwest Nigeria. Parasites from a total of 210 of 360 blood samples diagnosed with malaria were studied; DNA was extracted and amplified in nested Polymerase Chain Reaction (PCR) with primers specific for Plasmodium falciparum multidrug resistant (Pfmdr1) and the Kelch propeller (K13) genes. Amplicons were resolved on a 2% agarose gel and 5μl secondary amplicon were digested using restriction enzymes AflIII to detect polymorphism on codon 86 in the Pfmdr1 gene or sequenced to detect SNPs in the genes from few isolates (10%). The result of this study showed high prevalence (100%) of Pfmdr1 86Tyr mutant allele observed in all of the 4 isolates sequenced. For K13 gene, 13 unique mutations that have not been previously reported to be associated with artemisinin resistance were observed from one of the isolates but no marker of AR was observed. In conclusion, parasite isolates from this endemic area harbor high Pfmdr1 mutations and other mutations on K13 with unknown consequences, which may influence clinical responses to artemisinin and its partner drug.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Engineering, Science and Mathematics > School of Chemistry |
| Depositing User: | Mrs Hannah Akinwumi |
| Date Deposited: | 04 Feb 2020 10:46 |
| Last Modified: | 04 Feb 2020 10:46 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/13097 |
Actions (login required)
 |
View Item |
