Cytotoxic Effects of Compounds Isolated from Ricinodendron heudelotii

Yakubu, Omolara Faith and Adebayo, H. A. and Dokunmu, Titilope M. and Zhang, Ying-Jun and Iweala, E. E. J. (2019) Cytotoxic Effects of Compounds Isolated from Ricinodendron heudelotii. Molecules.

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Abstract

This study was designed to explore the in vitro anticancer effects of the bioactive compounds isolated from Ricinodendron heudelotii on selected cancer cell lines. The leaves of the plant were extracted with ethanol and partitioned in sequence with petroleum ether, ethyl acetate, and n-butanol. The ethyl acetate fraction was phytochemically studied using thin layer chromatography (TLC) and column chromatography (CC). Structural elucidation of pure compounds obtained from the ethyl acetate fraction was done using mass spectra, 1H-NMR, and 13C-NMR analysis. The isolated compounds were subsequently screened using five different cancer cell lines: HL-60, SMMC-7721, A-549, MCF-7, SW-480, and normal lung epithelial cell line, BEAS-2B, to assess their cytotoxic effects. Nine compounds were isolated and structurally elucidated as gallic acid, gallic acid ethyl ester, corilagin, quercetin-3-O-rhamnoside, myricetin-3-O-rhamnoside, 1,4,6-tri-O-galloyl glucose, 3,4,6-tri-O-galloyl glucose, 1,2,6-tri-O-galloyl glucose, and 4,6-di-O-galloyl glucose. Corilagin exhibited the most cytotoxic activity with an IC50 value of 33.18 �g/mL against MCF-7 cells, which were comparable to cisplatin with an IC50 value of 27.43 �g/mL. The result suggests that corilagin isolated from R. heudelotii has the potential to be developed as an effective therapeutic agent against the growth of breast cancer cells.

Item Type:	Article
Uncontrolled Keywords:	anticancer; Ricinodendron heudelotii; chemoprevention
Subjects:	Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	Mrs Patricia Nwokealisi
Date Deposited:	10 Mar 2020 10:41
Last Modified:	10 Mar 2020 10:41
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/13180

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