LAWAL, Beloved John and Covenant University, Theses (2018) PECTINASE PRODUCTION BY Aspergillus niger USING PECTIN FROM PINEAPPLE PEELS AND ITS APPLICATION IN COCONUT OIL EXTRACTION. Masters thesis, COVENANT UNIVERSITY.
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Abstract
Pectinases are a group of enzymes that hydrolyze pectic substances. This research is designed to produce pectinase from A. niger using laboratory produced pectin and to apply the pectinase produced to the extraction of oil from coconut. A. niger was isolated from five days old ‘eba’. The isolate was identified by morphological characteristics. The identified isolate was inoculated into basal salt medium and pectinase was produced by submerged fermentation over a seven-day period at a 24h interval. The extracellular pectinase was partially purified using activated charcoal and characterized using such parameters as effect of temperature, effect of time of heating, effect of substrate concentration and effect of metal ions. Then, the crude and partially purified were applied to the extraction of oil from coconut. Process optimization was done with central composite design using Response surface methodology (RSM). The highest pectinase activity was obtained on the fifth day of incubation. Optimum conditions for temperature, pH and substrate concentration were ascertained at 400C, 5 and 2 % respectively for pectinase produced by A. niger. The enzyme lost all its activity within 30min of heating at 900C and metal ion (Mg2+) stimulated the activity. This study revealed the production of pectinase from Aspergillus niger, optimized the process for industrial production of the enzyme and revealed its effectiveness for extraction of oil.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | Mrs Hannah Akinwumi |
| Date Deposited: | 26 Mar 2020 11:03 |
| Last Modified: | 26 Mar 2020 11:03 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/13267 |
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