Peter-Albert, C. F. and Covenant University, Theses (2013) STUDIES ON XYLANASE PRODUCTION BY Aspergillus niger ON TOMATO POMACE MEDIUM. ["eprint_fieldopt_thesis_type_phd" not defined] thesis, Covenant University.
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Abstract
Xylanase production by Aspergillus niger was investigated using tomato pomace medium in comparison with a basal salt medium. The importance of xylanase in industries cannot be overemphasised. They are used for baking, bleaching paper pulp, improving animal feed, bioethanol production, fruit juice and beer clarification to mention a few. Considering the vast importance of xylanase enzyme, there is a need for locally produced xylanase enzyme. Aspergillus niger was obtained from deteriorated banana (Musa acuminata) fruit. A pure culture was obtained and prepared on agar slants. Tomato pomace medium and a basal salt medium were inoculated with 72-h-old culture of Aspergillus niger. Xylanase production was tested in both growth media after four days of inoculation at room temperature (27 0C). Xylanase activity was determined by measuring the released reducing sugar (xylose) and the specific activities of xylanase from the basal salt medium and the tomato pomace medium were 3.6 U/mg and 2.0 U/mg respectively. Partial purification of the enzyme was carried out by Ammonium sulphate precipitation. The optimum condition for xylanase activity was determined by characterizing the partially purified enzyme. Optimum temperature and pHof 40oC and 3.5 respectively was obtained for the partially purified enzyme. Optimum substrate concentration of 0.5mg/ml and a purification fold of 4.3 were obtained for the xylanase. The apparent dissociation constant or Michaelis Menten constant (Km) which is the substrate concentration at half maximum velocity obtained from the Line-weaver burk plot was approximately 0.50mg/ml. This study established that the fungal strain of A. niger used for this study produced appreciable xylanase activity. This strain is a potential organism for the utilization of tomato waste. This makes tomato wastes a suitable medium for fungal xylanase production. The use of tomato waste will cut down on the foreign exchange spenton importation of xylanase by industries that depend on its use for their manufacturing processes.
| Item Type: | Thesis (["eprint_fieldopt_thesis_type_phd" not defined]) |
|---|---|
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | Mrs Hannah Akinwumi |
| Date Deposited: | 16 Nov 2020 15:52 |
| Last Modified: | 16 Nov 2020 15:52 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/13729 |
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