Adelani, Isaacson and Ogadi, E. O. and Onuzulu, Chinonye and Rotimi, Oluwakemi A. and Maduagwu, E. N. and Rotimi, S. O. (2020) Dietary vitamin D ameliorates hepatic oxidative stress and inflammatory effects of diethylnitrosamine in rats. Heliyon, 6 (9).
Full text not available from this repository.Abstract
The generation of reactive oxygen species (ROS) plays an essential role in the pathogenesis of several diseases. Its implication in inflammation has suggested a possible link between oxidative stress and activation/release of cytokines in precancerous states. Recent observational studies have suggested an association between inflammation and vitamin D deficiency; hence, suggesting that vitamin D could play a role in the pathogenesis of diseases. This study examined the antioxidant and anti-inflammatory potentials of vitamin D in diethylnitrosamine (DEN)-induced oxidative stress and inflammation in rats. Rats were divided into four experimental groups. While groups one and two were administered twice weekly with 30 mg/kg body weight DEN for six weeks, groups three and four were given normal saline. Groups one and three were fed with vitamin D deficient diet, while groups two and four were fed vitamin D diet during the experiment. After that, biomarkers of oxidative stress status were assayed spectrophotometrically. The concentration of inflammatory cytokines was determined using enzyme-linked immunosorbent assay (ELISA). DEN-induced vitamin D deficient diet group had increased antioxidant enzymes’ activities. Also, there were elevated concentrations of thiobarbituric acid reactive substances (TBARS) and inflammatory cytokines in the same group. Vitamin D diet, however, reduced oxidative stress effects through the reduction in the activities of TBARS and caused a significant (p < 0.05) increase in nitric oxide concentration. Vitamin D diet significantly (p < 0.05) reduced the level of interleukin 1β and TNF-α produced in the deficiency state. These findings show that vitamin D may play an essential role in the regulation of hepatic oxidative stress and inflammatory responses.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Nutrition Biochemistry Toxicology DietVitamin D Antioxidant Oxidative stress Inflammation Anti-inflammation Diethylnitrosamine |
| Subjects: | Q Science > Q Science (General) Q Science > QP Physiology |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | Oluwakemi A. Rotimi |
| Date Deposited: | 13 May 2021 08:13 |
| Last Modified: | 13 May 2021 08:13 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/14038 |
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