Vitamin K protects against 7,12-dimethylbenz(A)anthracene induced hepatotoxicity in Wistar rats

Dosumu, O.A. and Rotimi, S.O. and Adeleye, O.O. and Akamo, A.J. and Osinuga, K.T. and Taiwo, O.A. and Omotosho, O.O. and Sani, L.O. (2021) Vitamin K protects against 7,12-dimethylbenz(A)anthracene induced hepatotoxicity in Wistar rats. Environmental Toxicology, 36 (3). pp. 362-373.

Other (na) Download (117kB)

Abstract

Humans are daily exposed to 7,12-dimethylbenz(a)anthracene (DMBA), a well known polycyclic aromatic hydrocarbons (PAH). This study investigated the role of dietary intake of Vitamin K (VK), a polyphenolic compound, with potential antioxidative properties, against DMBA-induced hepatotoxicity. Sixty experimental animals (120-150 g) were divided into six groups (A-F): Control, DMBA (80 mg/kg bw) only, VK (0.00 g/10 kg) diet only, VK (7.5 g/10 kg) diet only, DMBA + VK (0.0 g/10 kg) diet and DMBA + VK (7.5 g/10 kg) diet. Single oral administration of DMBA (80 mg/kg body weight) to Wistar rats resulted in hepatic damage after 16 weeks. DMBA significantly (P <.05) decreased the activities of catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST) and glutathione peroxidase (GPx). Levels of reduced glutathione (GSH) and Vitamin C were significantly decreased with increase in malondialdehyde (MDA) and nitric oxide (NO) levels in serum and liver. Aspartate aminotransaminase (AST), alanine aminotransaminase (ALT), γ-glutamyltransferase (GGT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) activities were significantly (P <.05) elevated in the serum but reduced in the liver of DMBA-administered group. Ingestion of 7.5 g/10 kg VK diet prevented the up regulations in inflammatory biomarkers (granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin 17A (IL-17A)) which elicited liver damaged in the DMBA-treated group. DMBA induced hepatic alterations in DMBA-treated group but was restored to near normal in VK (7.5 g/10 kg) diet group. These findings suggest the protective potential of increased dietary intake of vitamin K against DMBA-induced hepatic dysfunction. © 2020 Wiley Periodicals LLC.

Item Type:	Article
Additional Information:	cited By 0
Uncontrolled Keywords:	Amino acids; Anthracene; Nitric oxide; Nutrition; Peptides; Phosphatases; Plants (botany); Polyphenolic compounds; Rats, Antioxidative property; Gamma-glutamyltransferase; Glutathione peroxidase; Glutathione S-transferases; Granulocyte-macrophage colony stimulating factors; Lactate dehydrogenase activities; Polycyclic aromatic hydrocarbon (PAH); Superoxide dismutases, Macrophages, 7,12 dimethylbenzaanthracene; alanine aminotransferase; alkaline phosphatase; ascorbic acid; aspartate aminotransferase; bilirubin; biological marker; catalase; gamma glutamyltransferase; glutathione; glutathione peroxidase; glutathione transferase; granulocyte macrophage colony stimulating factor; interleukin 17; ketamine; lactate dehydrogenase; malonaldehyde; nitric oxide; superoxide dismutase; vitamin K group; xylazine; anthracene derivative; antioxidant; ascorbic acid; catalase; glutathione; glutathione peroxidase; glutathione transferase; superoxide dismutase; vitamin K group, antioxidant; dietary intake; ecotoxicology; induced response; PAH; rodent; vitamin, alanine aminotransferase blood level; alkaline phosphatase blood level; animal cell; animal experiment; animal model; animal tissue; antioxidant activity; Article; aspartate aminotransferase blood level; bilirubin blood level; blood sampling; cell infiltration; comparative study; controlled study; dietary intake; enzyme activity; female; gamma glutamyl transferase blood level; histopathology; inflammation; ingestion; lactate dehydrogenase blood level; lipid peroxidation; liver function; liver function test; liver histology; liver homogenate; liver injury; liver protection; liver tissue; liver toxicity; nonhuman; priority journal; protein blood level; rat; upregulation; vitamin supplementation; animal; drug effect; liver; male; metabolism; oxidative stress; toxic hepatitis; Wistar rat, Rattus norvegicus, 9,10-Dimethyl-1,2-benzanthracene; Animals; Anthracenes; Antioxidants; Ascorbic Acid; Catalase; Chemical and Drug Induced Liver Injury; Glutathione; Glutathione Peroxidase; Glutathione Transferase; Liver; Male; Oxidative Stress; Rats; Rats, Wistar; Superoxide Dismutase; Vitamin K
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisions:	UNSPECIFIED
Depositing User:	Solomon Rotimi
Date Deposited:	18 Jun 2021 16:00
Last Modified:	18 Jun 2021 16:00
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/14618

Actions (login required)

View Item