GC-MS Analysis and Inhibitory Evaluation of Leaf Extracts on Major Enzymes Linked to Diabetes.

Iheagwam, Franklyn Nonso and Israel, Emmanuel Nsedu and Kayode, Kazeem Oyindamola and De Campos, Opeyemi Christianah and Ogunlana, Olubanke Olujoke and Chinedu, Shalom Nwodo (2019) GC-MS Analysis and Inhibitory Evaluation of Leaf Extracts on Major Enzymes Linked to Diabetes. Evidence-based complementary and alternative medicine : eCAM, 2019. p. 6316231. ISSN 1741-427X

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Abstract

leaves are used in managing both diabetes mellitus and its complications in Southwest Nigeria. However, its inhibitory activity on enzymes implicated in diabetes is not very clear. This study investigated the inhibitory properties and mode of inhibition of leaf extracts on enzymes associated with diabetes. The study also identified some bioactive compounds as well as their molecular interaction in the binding pocket of these enzymes. Standard enzyme inhibition and kinetics assays were performed to determine the inhibitory effects of aqueous extract (TCA) and ethanol extract (TCE) of leaves on -glucosidase and -amylase activities. The phytoconstituents of TCA and TCE were determined using GC-MS. Molecular docking of the phytocompounds was performed using Autodock Vina. TCA and TCE were the most potent inhibitors of -glucosidase (IC = 3.28 ± 0.47 mg/mL) and -amylase (IC = 0.24 ± 0.08 mg/mL), respectively. Both extracts displayed a mixed mode of inhibition on -amylase activity, while mixed and noncompetitive modes of inhibition were demonstrated by TCA and TCE, respectively, on -glucosidase activity. The GC-MS analytic chromatogram revealed the presence of 24 and 22 compounds in TCE and TCA, respectively, which were identified mainly as phenolic compounds, terpenes/terpenoids, fatty acids, and other phytochemicals. The selected compounds exhibited favourable interactions with the enzymes compared with acarbose. Overall, the inhibitory effect of on -amylase and -glucosidase may be ascribed to the synergistic action of its rich phenolic and terpene composition giving credence to the hypoglycaemic nature of leaves.

Item Type:	Article
Subjects:	Q Science > Q Science (General)
Divisions:	UNSPECIFIED
Depositing User:	Franklyn N IHEAGWAM
Date Deposited:	20 Jun 2021 21:49
Last Modified:	20 Jun 2021 21:49
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/14761

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