Thaumatococcus daniellii (Benn.) Benth, a rainforest berry of West Africa produces fruits containing a black, hard, impervious seed surrounded by a jelly coat. The seeds are reported to have some therapeutic effects. This study examined the effect of ethanolic seed extract of T. daniellii on lipid profile in male Wistar rats. Ethanolic extract of T. daniellii seeds was prepared and graded concentrations were administered orally to the rats for 14 days. Blood, heart, brain and testes were excised and lipid parameters where assayed. Result were statistically analysed using one way analysis of variance (ANOVA) supplemented with Duncan multiple range test (DMRT). There was a significant (p<0.05) decrease in cholesterol and triglyceride concentration and a significant increase (p<0.05) in HDL-cholesterol concentration compared to the normal and positive control. There was no significant (p<0.05) difference in animal weight, organ weight and LDL-cholesterol concentration. The hypolipidemic potential of the seed extract can be exploited for the management and prevention of cardiovascular disease.