Phylogenetic relationship and genetic variation among thaumatococcus daniellii and megaphrynium macrostachyum ecotypes in southwest Nigeria

Chinedu, S.N. and Emiloju, O.C. and Iheagwam, Franklyn N and Rotimi, S.O. and Popoola, J.O. (2018) Phylogenetic relationship and genetic variation among thaumatococcus daniellii and megaphrynium macrostachyum ecotypes in southwest Nigeria. Asian Journal of Plant Sciences, 17 (1). pp. 27-36.

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Abstract

Background and Objective: Thaumatococcus daniellii (T. daniellii) and Megaphrynium macrostachyum (M. macrostachyum) are perennial, understorey herbs with similar morphological and vegetative properties. Genetic relatedness and variation among and within ecotypes of the plant species in Nigeria were studied using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Materials and Methods: Plant samples were collected at random from 24 sites across 5 states in Southwest Nigeria. The DNA was extracted from the leaves by cetyl trimethyl ammonium bromide (CTAB) method and amplified with respective PCR-based primers. Genetic variation within and between the populations was determined by analysis of molecular variance (AMOVA), cluster analysis was performed using unweighted pair group method using arithmetic averages (UPGMA). Results: The RAPD and ISSR markers generated clear reproducible bands, polymorphic bands (PPD) produced by RAPD and ISSR markers were 86.7 and 91.3, respectively. Genetic variation among the two plant populations was 93.68 and 77.87, respectively with RAPD and ISSR markers, variation within each plant population was 6.32 and 22.13, respectively for the RAPD and ISSR primers. The cluster analysis indicated that genetic differentiation occurred more among the population than within population of T. daniellii and M. macrostachyum. Conclusion: This study showed that T. daniellii and M. macrostachyum species are genetically distinct from each other. However, the genetic variation within each of the two species is low, thus, management and conservative approaches are required to preserve and develop the plants for future commercial exploitation. © 2018 Shalom Nwodo Chinedu et al.

Item Type:	Article
Additional Information:	cited By 2
Subjects:	Q Science > Q Science (General)
Divisions:	UNSPECIFIED
Depositing User:	Franklyn N IHEAGWAM
Date Deposited:	21 Jun 2021 16:35
Last Modified:	21 Jun 2021 16:35
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/14794

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