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Establishing surface sterilization protocol for nodal culture of Solanecio biafrae.

Bello, Oluwakemi A. and Esan, E. B. and Obembe, Olawole O. (2018) Establishing surface sterilization protocol for nodal culture of Solanecio biafrae. In: 8th International Biotechnology Conference, Exhibition and Workshop, 2018, Online.

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Surface sterilization of plant materials is a very problematic step in establishing plant tissue culture protocol. Prior to introduction into sterile Murashige and Skoog (MS) media, nodal explants obtained from potted plants of Solanecio biafrae grown in the screenhouse were given various surface sterilization treatments. A total of 16 treatments with varying time of immersion in two sterilant successions, 70% (v/v) ethanol (20 s, 1 min, 3 min and 5 min) and 10% (w/v) calcium hypochlorite (Ca(ClO)2) granules (5, 10, 15 and 20 min) at varying times and then rinsed three times with sterile distilled water. 100% clean culture was obtained from treatments containing 70% ethanol (20 s)+10% Ca(ClO)2 (15 min) and 70% ethanol (3 min)+10% Ca(ClO)2 (20 min) after 4 weeks in culture. 70% ethanol (20 s)+10% Ca(ClO)2 (15 min) is proposed as the best surface sterilization protocol as it gave 90% growth compared to 60% growth recorded in 70% ethanol (3 min)+10% Ca(ClO)2 (20 min). This is a preliminary study as more research is ongoing to establish an efficient, effective and reproducible protocol for the surface sterilization of different explants used for the tissue culture of S. biafrae obtained from open field

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Murashige & Skoog, calcium hypochlorite (Ca(ClO)2, surface sterilization, nodal explant, Solanecio biafrae
Subjects: Q Science > QH Natural history
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mrs Patricia Nwokealisi
Date Deposited: 12 Aug 2021 15:37
Last Modified: 12 Aug 2021 15:37

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