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Antimicrobial activity and time kill kinetics of Nigerian Honeys on multi-resistant Enteric Bacilli

Akinduti, P. A. and OLUWADUN, AFOLABI and OLUGBUYIRO, J.A.O and Osuagwu, C. S. and Ejilude, Oluwaseun and Onagbesan, K. O. and Olasehinde, G. I and Taiwo, O. S. and Obafemi, Yemisi Dorcas (2018) Antimicrobial activity and time kill kinetics of Nigerian Honeys on multi-resistant Enteric Bacilli. In: 8th International Biotechnology Conference, Exhibition and Workshop, 2018, Online.

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Antimicrobial activities of some Nigeria honeys were tested on multi-antibiotic resistant enteric bacilli strains (MAREBS) that are becoming dreadful among the populace. Enteric bacilli isolated from fecal samples randomly collected from community populace were biotyped and profiled for antibiotic susceptibility by micro-broth dilution assay. Honey physico-chemical and phyto-chemical metabolites were analysed and tested for antimicrobial susceptibility to MAREBS while its time kill kinetics was evaluated. Significant rate of 31.3% Escherichia coli, Klebsiella oxytoca, (19.5%), Pseudomonas aeruginosa (15.3%) were found with only 62.6% showed significant resistance to cefotaxime (30μg) and 61.6% to ampicillin (10μg). more than 40% showed significant resistance to Cotrimoxazole, ciprofloxacin and tetracycline with MIC >16 μg/ml (p<0.05). Physico-chemical parameters vary significantly with high phenol and alkaloids contents. Few honey samples showed antimicrobial activity of more than 37% inhibition rate while 8.1% MAREBS were further inhibited at lower MIC 31.25mg/mL, 10.8% at MIC 125mg/mL and 8.1% MIC 250mg/mL, while cidal rate of 8.1% was recorded. Significant reduction in average count of different MAREBS was recorded at honey dilutions of 1:2 and 1:4 to less than 2.10Log10CFU/mL. Amidst global burden of enteric infection with persistence antibiotic resistance, Nigerian honeys showed a reliable bacteriostatic and cidal activity as prospective novel alternative therapy for MAREBS infections.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: antibiotics, resistant, honey, phytochemicals, physico-chemicals
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Divisions: Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User: Mrs Patricia Nwokealisi
Date Deposited: 01 Sep 2021 10:07
Last Modified: 01 Sep 2021 10:07

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