EVBUOMWAN, SARAH ADESUWA and Covenant University, Theses (2021) EFFECTS OF Cnidoscolus aconitifolius LEAF EXTRACTS ON MONOSODIUM GLUTAMATE (MSG)-INDUCED OXIDATIVE STRESS IN RATS. Masters thesis, COVENANT UNIVERSITY.
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Abstract
Monosodium glutamate (MSG) is found naturally in some foods. MSG is used in cooking as a flavour enhancer with an umami taste that intensifies the flavour of food. MSG has been linked with several diseases such as metabolic disorders, Chinese restaurant syndrome, neurotoxic effects, oxidative stress and reproductive toxicity. The aim of this research was to assess the effect of Cnidoscolus aconitifolius leaf extracts on the oxidative stress intake of MSG using antioxidant enzyme activity, assessment of liver and kidney functions, histopathological parameters and gene expression analysis. Forty five animals were used for this study and were assigned into nine (9) experimental groups of 5 animals each. Group 1 was given 0.9% normal saline, groups 2 and 3 were given only MSG and extract respectively, while groups 4 and 5 were pre- and post-treated with the extract before and after the intake of MSG for four (4) weeks. The MSG doses given were 2.4g/kg body weight while 200mg/kg and 400mg/kg of extracts were administered respectively. All treatments were carried out for a period of eight (8) weeks (56 days). The animals were sacrificed after the stated period of administration with collection of blood and organs for assays. Assays such as assessment of Liver and Kidney function, antioxidant enzyme activity and gene expression analysis were carried out. Results showed that there was a significant increase (P<0.05) in the body weights, Aspartate amino transferase (AST) activity and creatinine concentration of the rats given only MSG compared with the controls. Alanine amino transferase (ALT) activity and urea concentration was also increased in the MSG group. The content of the phytochemicals in the showed high value in saponin (0.28± 0.002mg/ml), followed by flavonoids (0.17±0.007mg/ml) which might give the plant the effect to modulate the negative effects of the MSG. The extracts also improved the histologic structure of the rat organs compared to the groups given MSG which had severe necrosis and degeneration of their cells. Administration of MSG downregulated the expression of Superoxide dismutase (SOD) and Catalase (CAT) genes in the liver and kidneys when compared to the control group. It can therefore be concluded that Cnidoscolus aconitifolius has some bioactive properties with antioxidant potentials which need to be fully explored
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Monosodium glutamate, Cnidoscolus aconitifolius, Antioxidants, Oxidative stress, Gene expression |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | Mrs Hannah Akinwumi |
| Date Deposited: | 11 Jan 2022 10:53 |
| Last Modified: | 11 Jan 2022 10:53 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/15556 |
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