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Emerging vancomycin-non susceptible coagulase negative Staphylococci associated with skin and soft tissue infections

Akinduti, P. A. and Obafemi, Yemisi Dorcas and Ugboko, U. H. and El‑Ashker, Maged and Akinnola, Olayemi O. and Agunsoye, Chioma Jane and Oladotun, Abiola and Phiri, Bruno S. J. and Oranusi, S. U. (2022) Emerging vancomycin-non susceptible coagulase negative Staphylococci associated with skin and soft tissue infections. Annals of Clinical Microbiology and Antimicrobials, 21 (31). pp. 1-10.

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Backgrounds: Observable emergence of Vancomycin-Non susceptible Coagulase-negative Staphylococci (VNSCoNS) associated with skin and soft tissue infections spreading among the urban and rural populace is gradually intensifying severe complications. The isolated VNS-CoNS were evaluated with Matrix-assisted Laser Desorption/ioni‑ zation Time of Flight Mass Spectrometry (MALDI ToF MS) for species characterization and pan-antimicrobial resistance pattern. Methods: Out of 256 clinical samples collected including pus, abscess, ear swabs, eye swabs, and aspirates, 91 CoNS isolates were biotyped and further characterized with MALDI-TOF MS. Staphylococci marker genes, Vancomycin sus‑ ceptibility, and bioflm assays were performed. Results: Of 91 CoNS isolates, S.cohnii (2.3%), S.condimentii (3.4%), S. saprophyticus (6.7%), and S.scuri (21.1%) were characterized with MALDI-TOF with signifcant detection rate (99.4%; CI 95, 0.775–0.997, positive predictive values, 90.2%) compared to lower biotyping detection rate (p=0.001). Hemolytic VNS-CoNS lacked nuc, pvl and spa genes from wound, ear, and aspirates of more 0.83 MARI clustered into a separate phylo-diverse group and were widely distributed in urban and peri-urban locations. MALDI TOF–MS yielded a high discriminatory potential of AUC-ROC score of 0.963 with true-positivity prediction. VNS-CoNS of MIC≥16 µg/mL were observed among all the ages with signifcant resistance at 25th and 75th quartiles. More than 10.5% of CoNS expressed multi-antibiotic resistance with more than 8 µg/mL vancomycin cut-of values (p<0.05). Conclusion: Antibiotic resistant CoNS should be considered signifcant pathogens rather than contaminant. Bioflm producing VNS-S. sciuri and S. condimentii are potential strains with high pathological tropism for skin, soft tissues and wound infections, and these strains require urgent surveillance in peri-urban and rural communities.

Item Type: Article
Uncontrolled Keywords: Coagulase-negative staphylococci, Antibiotic, Communities, Resistance, Vancomycin
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mrs Hannah Akinwumi
Date Deposited: 12 Jul 2022 14:00
Last Modified: 12 Jul 2022 14:00

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