Okesola, Mary Abiola and Covenant University, Theses (2022) BIOCHEMICAL AND GENE EXPRESSION STUDIES ON UTERINE FIBROID PATIENTS IN IBADAN, OYO STATE, NIGERIA. ["eprint_fieldopt_thesis_type_phd" not defined] thesis, Covenant University Ota.
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Abstract
Uterine fibroids (UF) are benign, non-carcinomatous growths that reside in the smooth layer of the uterus and cause many gynaecological problems among reproductive-age women worldwide. Their occurrence infringes on women's biological, sociological, physiological status and quality of lifestyle with an added financial burden, especially in black women. The actual cause of the phenomenon remains undiagnosed despite numerous studies, thereby prompting further investigation on its relationship to some biochemical and molecular markers of cellular growth and development. One hundred and ninety (190) reproductive-aged women (95 each for UF patients and age-matched non-UF participants) were recruited for the study. The experimental design was divided into two phases - Case-control model, and Case-control age-related model [16-25 years (group A), 26-35 years (group B), 36-45 years (group C), 46-55 years (group D) and 56 & above (group E)]. A portion of 5g each of the excised fibroid tissues and normal tissue samples from the adjacent myometrium were collected and sectioned into three parts. The first part was preserved using RNAlater® and kept at -80⁰ C for molecular analysis. The second portion was preserved with 10 % formalin solution for histological examination, while the last portion was rinsed with normal saline and frozen for biochemical analysis. The blood samples (10mL) were also collected; a 2mL portion was used for haematological analysis and the remaining 8mL for biochemical tests. Vitamins A, D, and K concentrations, the female reproductive hormones, and inflammatory markers (oestrogen, progesterone, IL-1, IL-2, CoX-1, CoX-2, and TNF-α respectively) were assessed using Enzyme-linked immunosorbent assay kits. Concentrations of mineral elements in UF and non-UF were examined using atomic absorption spectrophotometry. The supernatant obtained from tissues and serum was utilised to assess the antioxidant status, and vitamins E and C concentrations were investigated spectrophotometrically. Age, reproductive hormones, antioxidant enzymes and micronutrients were identified as contributing factors promoting the incidence or occurrence of the UF. The micronutrient examination in the phase one design model revealed that selenium of all the examined mineral elements and vitamins A, D, and E were significantly reduced while Vitamin K increased significantly in UF cases at (p<0.05) promoting the occurrence of tumour. There was a significant increase (p<0.05) in the level of vitamin K, sodium, magnesium, iron, zinc and IL-2 in group C; Ca in group E, progesterone in groups B and C, reduction of progesterone in group D, estrogen, and cholesterol in group D; the activities of glutathione-s-transferase and over expression of transforming growth factor gene in the fibroid tissues. Conversely, there was a significant reduction of (P<0.05) in the levels of reduced glutathione in groups B, C and E, and SOD and selenium in groups E and D respectively. Similarly, there was a significant (P<0.05) decrease in the levels of vitamin A in groups B and C, vitamin D in groups D, vitamin E in groups D and E, sodium and cholesterol in group B in UF cases. The UF incidence or occurrence were associated with the nutritional influence of vitamin E, A = D, and selenium deficiencies in decreasing order, excess vitamin K intake and with overexpression of TGF-β. Thus, biochemical and molecular processes related to dietary lifestyle are strongly associated factors facilitating the occurrence of UF in most Black women.
| Item Type: | Thesis (["eprint_fieldopt_thesis_type_phd" not defined]) |
|---|---|
| Uncontrolled Keywords: | Antioxidant, ELISA Kits, Gene expression, Inflammation, Uterine fibroids, Vitamins. |
| Subjects: | Q Science > QH Natural history |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | nwokealisi |
| Date Deposited: | 22 Nov 2022 14:57 |
| Last Modified: | 22 Nov 2022 14:57 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/16467 |
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