In vitro and in-silico inhibitory validation of Tapinanthus cordifolius leaf extract on alpha-amylase in the management of type 2 diabetes

Chike-Ekwughe, Amarachi and Adegboyega, Abayomi Emmanuel and Johnson, Titilayo Omolara and Adebayo, H. A. and Ogunlana, Olubanke Olujoke (2023) In vitro and in-silico inhibitory validation of Tapinanthus cordifolius leaf extract on alpha-amylase in the management of type 2 diabetes. Informatics in Medicine Unlocked.

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Abstract

Tapinanthus cordifolius an African mistletoe is an important medicinal plant that has been shown to lower postprandial hyperglycemia. It has been proposed that mistletoe’s hypoglycemic effects are related to its ability to lower blood glucose levels and that the anti-diabetic activity of some of these parasitic plants might be due to their ability to block the action of alpha-amylase. To identify prospective alpha-amylase inhibitors for anti- diabetic drug discovery, the current study used in vitro experiments and computational methods such as mo- lecular docking, pharmacophore modelling, and ADMET profiling to evaluate the alpha-amylase inhibitory ability of Tapinanthus cordifolius leaf extracts and its bioactive components. The crude extract of the plant showed the highest inhibiting activity with an IC50 value of 26.88 μg/ml. Gas chromatography-mass spectroscopic analysis of the extract gave 43 phytochemicals, including sesquiterpenes, diterpenes, triterpenes and their de- rivatives, phytosterols, and tocopherols. The molecular docking analysis of these compounds with alpha-amylase identified benzaldehyde, 4-(Ethylthio)-2,5-dimethoxy, alpha-Tocopherol-Beta-D-Mannoside, 5-ergosterol, 3,4,5- trimethoxybenzoic acid, and acetosyringone as the five top-scoring compounds. Their binding energies which ranged from

Item Type:	Article
Uncontrolled Keywords:	Tapinanthus cordifolius Hyperglycemia Alpha amylase Computational methods Molecular docking Benzaldehyde 4-(Ethylthio)-2 5-Dimetho
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	AKINWUMI
Date Deposited:	25 Apr 2023 22:15
Last Modified:	25 Apr 2023 22:15
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/16847

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