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Isolation and Screening of Laccase-producing Fungi from Sawdust-contaminated Sites in Ado-Odo Ota, Ogun State, Nigeria

Akinyemi, Oluwatobi D. and Ahuekwe, Eze Frank and Akinduti, P. A. and Oziegbe, Olubukola and Oniha, M. I. and Ichor, T. and Alade, B. M. and Ezekiel, O. M. and Adekeye, B. Temitope and Kuye, Alice O. (2022) Isolation and Screening of Laccase-producing Fungi from Sawdust-contaminated Sites in Ado-Odo Ota, Ogun State, Nigeria. In: 3rd International Conference on Energy and Sustainable Environment, 2022, Online.

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The environmental imbalance exerted by the continuous release of phenolic substances necessitates a return of polluted sites to natural and safe status. In this study, fungal isolates obtained from sawdust-contaminated soils were screened for laccase production capacities, using tannic acid, as an index to the bio-stimulatory potentials of the sawdust. Soil and sawdust samples collected from wood-processing plants in Morogbo-Agbara (M), Iju (I), and Oja (O) of Ado-Odo/Ota, Ogun State, Nigeria were subjected to physicochemical analysis. The phenolic content estimated using gallic acid calibration curve, showed 0.90%, 0.79% and 0.33% for the soil samples labeled MSL, ISL, OSL, respectively. Phenol content was observed to be 0.63%, 0.91%, and 0.53% for sawdust samples labeled MSD, ISD, OSD, respectively. In the same labeling order, the percentage nitrogen content was 0.77%, 0.38%, and 0.21% for soil; and 0.0025%, 0.0035% and 0.0028% for sawdust; while the percentage carbon was 0.25%, 0.62% and 0.49% for soil samples; and 88.11%, 85.56%, and 88.69% for the sawdust samples. Fungal species of Aspergillus, Penicillium, Candida and Saccharomyces among the ten isolates presented a positive reaction for laccase production by showing a brownish-black coloration. The ability of the fungal isolates to produce laccase makes them useful laccase sources for industrial and environmental applications

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Sawdust, laccase, fungi, soil, phenolic content.
Subjects: Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: nwokealisi
Date Deposited: 02 May 2023 12:42
Last Modified: 02 May 2023 12:42

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