Activities of Acetylcholinesterase, Oxidative and Nitrosative Stress Markers in Clarias gariepinus Exposed to ‘Uproot’, a Glyphosate–Based Herbicide

Osioma, E. and Ejoh, Akpoyovware S. (2021) Activities of Acetylcholinesterase, Oxidative and Nitrosative Stress Markers in Clarias gariepinus Exposed to ‘Uproot’, a Glyphosate–Based Herbicide. International Journal of Zoological Investigations, 7 (1). pp. 51-66. ISSN 2454-3055

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Abstract

In this study, Clarias gariepinus were exposed to different concentrations (60, 80 and 100 mg/L) of Uproot, a glyphosate-based herbicide and the activities of acetylcholinesterase (AChE), hepatic enzyme markers, oxidative and nitrosative stress markers were determined in serum, gills, liver and brain by using standard assays. Results showed that AChE activity was not significantly inhibited. Activities of ALT, AST and ALP increased (P < 0.05) wi th increasing exposures. For SOD, elevated (P < 0.05) activities were observed in all tissues at 60 mg/L exposure, but decreased at 80 mg/L and 100 mg/L concentrations. The activity of CAT reduced significantly (P < 0.05) in brain at 100 mg/L exposure. Increased production of NO was observed in gill and brain whereas in serum it is increased. GPx was elevated in gills and reduced in brain and liver. Gills showed lower MDA concentrations. GST was elevated in liver and brain, while total protein reduced (P < 0.05) in serum, gill and liver wi th increasing concentrations of exposure. The impaired activi ties of antioxidant enzymes and induction of NO suggest a disruption of normal antioxidant response in Clarias gariepinus exposed to glyphosate, and these activities could be used as biomarkers in aquatic environmental contamination.

Item Type:	Article
Uncontrolled Keywords:	Uproot, Glyphosate, Clarias gariepinus, Nitric oxide, Antioxidant enzymes
Subjects:	Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	nwokealisi
Date Deposited:	19 May 2023 09:37
Last Modified:	19 May 2023 09:37
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/16899

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