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Oxidative Stress Markers in Parasitized and Non-Parasitized Amietophrynus regularis (Reuss, 1833) Exposed to Multi- Stress Conditions in Lagos Metropolis, Nigeria

Okeagu, Okechukwu Martin and Akinsanya, Bamidele and Onadeko, Abiodun Benedict and Adekoya, Khalid and Isibor, Patrick Omoregie (2022) Oxidative Stress Markers in Parasitized and Non-Parasitized Amietophrynus regularis (Reuss, 1833) Exposed to Multi- Stress Conditions in Lagos Metropolis, Nigeria. Biology Bulletin, 49. S130-S138.

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Abstract

Background: Amphibians are subjected to a variety of natural and artificial stresses that can compromise physiological balance. This study explored the variations in oxidative stress markers in Cosmocercan parasitized and non-parasitized Amietophrynus regularis in diverse conditions. The toad specimens were collected from natural habitats and dumpsites of three (3) major areas of Lagos. 15 g wet weight of the liver, intestine, and parasites were separately prepared and analyzed for oxidative stress parameters including; SOD, CAT, GSH, and MDA activities according to well-established protocols. Result: Based on stations, there were significant differences in the SOD levels in the liver and intestine of infected and uninfected toads obtained at the dumpsite and the natural habitat at p < 0.001. Also, the concentration of SOD in the parasites (238.86 ± 7.72 min/mg prot.) recovered from the toads at the dumpsite was very much significantly higher than the concentration in those recovered from the toads in the natural habitat (p < 0.001). The study has shown that the level of SOD released by the antioxidant defense system in response to the oxidative stress in the organism makes it a reliable tool for ecotoxicological assessment. All the oxidative stress markers except for MDA showed significant differences at p < 0.05 between the host tissues and the parasites irrespective of sampling locations indicating both organisms were stressed either due to the host-parasite relationship or from other environmental contaminants. Conclusions: The toad’s antioxidant defense system was typically sensitive and adaptable to environmental conditions, suggesting that it could be used in future research on effective ecotoxicological evaluation tools.

Item Type: Article
Uncontrolled Keywords: dumpsite  oxidative stress  natural habitat  parasitized  antioxidant
Subjects: Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Engineering, Science and Mathematics > School of Engineering Sciences
Depositing User: nwokealisi
Date Deposited: 06 Oct 2023 13:33
Last Modified: 06 Oct 2023 13:33
URI: http://eprints.covenantuniversity.edu.ng/id/eprint/17360

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