AMUJI, DORIS NNENNA and Covenant University, Theses Masters (2024) EVALUATION OF SINGLE NUCLEOTIDE POLYMORPHISMS OF MESENCHYMAL EPITHELIAL TRANSITION GENE IN NIGERIAN BREAST CANCER PATIENTS. Masters thesis, Covenant University.
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Abstract
Breast cancer (BC) is a nationwide health issue, and understanding genetic risk factors is crucial for early detection and personalized treatment strategies. The Mesenchymal Epithelial Transition (MET) proto-oncogene, encoding the c-MET receptor tyrosine kinase (RTK), is key in BC progression and metastasis. This study identified single nucleotide polymorphisms (SNPs) of the MET gene, namely rs40239, rs1621, and rs41736, in Nigerian BC patients and their association with BC risk. The research involved 150 participants, including 75 BC patients and 75 healthy controls. DNA was extracted from participants' blood using the QIAamp DNA Mini Kit. The target SNPs (rs40239, rs1621, and rs41736) were genotyped on the DNA using the TaqMan SNP genotyping assay, and the allele frequencies were compared. An X2 test was employed to examine the association between the SNPs and BC risk. The results showed the presence of the SNPs with varying distributions in the participants. For MET rs40239, the AA genotype was more common in controls (60%) compared to patients (17%), though the association with BC risk was not statistically significant (OR=4.706, 95% CI: 0.959 – 23.096, p=0.062). For MET rs1621, no significant association with BC risk was found, with an odds ratio of 1.188 (95% CI: 0.516-2.733, p=0.426), and the genotype distribution showed minimal differences between controls and patients. Similarly, MET rs41736 also showed no significant association with BC risk (OR=0.954, 95% CI: 0.904 - 1.006, p=0.262), with genotype distributions showing minimal differences between groups. These findings suggest that while there were some differences in genotype distributions, none of the SNPs showed a statistically significant association with BC risk in this study. The results are crucial for understanding the genetic and functional implications of these MET gene variants of BC in the underrepresented African population. This would pave the way for future investigations that could influence strategies for early detection, diagnosis, and precision treatment of BC.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Breast cancer, Single nucleotide polymorphism, Genotype, Prognosis |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history R Medicine > RG Gynecology and obstetrics |
| Divisions: | Faculty of Engineering, Science and Mathematics > School of Chemistry |
| Depositing User: | Patricia Nwokealisi |
| Date Deposited: | 23 Sep 2024 15:13 |
| Last Modified: | 23 Sep 2024 15:13 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/18431 |
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