OYEGBADE, Samuel Adeniyi and Covenant University, Theses Masters (2024) GENETIC PROFILING OF K13 ARTEMISININ-RESISTANT Plasmodium falciparum AMONG PATIENTS ATTENDING HEALTHCARE FACILITIES IN OTA NIGERIA. Masters thesis, Covenant University.
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Abstract
Malaria stands out incessantly as a potential concern to public health systems in Africa, particularly with the prevalence of Plasmodium falciparum characterized with resistance to artemisinin alongside kelch13 (Pfkelch13) gene alterations. This study aimed to evaluate the prevalence of Plasmodium falciparum infection, genomic profiling and clonal diversity of PfKelch13 among patients attending selected health facilities in Ota, Nigeria. 479 patients with severe P. falciparum infection were engaged and their demographic status were collected. The blood samples from the patients were examined for P. falciparum infective stages and enumerated for parasitemia using Microscopy. DNA was isolated from high parasitemia P. falciparum samples and genotyped for Pfkelch13 using Primary and Nested PCR assays and examined with 1% agarose gel electrophoresis. Identified PfK13 samples were sequenced using Sanger sequencing method and further analyzed for clonal diversity. Overall, P. falciparum malaria identified by microscopy was 265 (55.33%). Significant prevalence of 33.61% was observed among 11-20 years age group, and 29.44% rates among male based on gender demography (p<0.05). Parasitemia levels (>200 parasites per 100uL) were higher among male than female populations and increases among age groups (0-10 and 11-20 years). Of 10.57% PfKelch13 genotypes, higher rates of 5.66% were observed among the male compared to female. Pf strains encoded with K13 from Nigerian population clustered with other global strains identified in UK, France, China and Kuwait. PfK13 strains obtained from this study clustered separately with strains previously reported in African countries including Ghana, Kenya and Nigeria. There is evidence of Pfkelch13 strains in Ota Southwest Nigeria having clonal relatedness with P. falciparum K13 markers Y494H and C580Y (Ghana), A578S (Kenya) and A675V (Nigeria). There is need for urgent geno-surveillance and public oriented preventive strategies to curb possible spread of PfK13 strains and identification of PfK13 markers for diagnosis and drug target.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Artemisinin-resistant Plasmodium falciparum, pfkelch 13, mutation, resistance, parasitemia |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH426 Genetics R Medicine > RA Public aspects of medicine |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | Patricia Nwokealisi |
| Date Deposited: | 30 Sep 2024 12:32 |
| Last Modified: | 30 Sep 2024 12:32 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/18470 |
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