Aririguzoh, Victoria-Grace O. and Covenant University, Theses Masters (2024) CHARACTERISATION OF Plasmodium falciparum PFMDR1 GENE MUTATIONS IN MALARIA PATIENTS FROM SELECTED HOSPITALS IN OTA, OGUN STATE, NIGERIA. Masters thesis, Covenant University.
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Abstract
Malaria continues to be a significant global health problem, especially in tropical and subtropical areas. The 2023 World Malaria Report by the World Health Organization reveals that there were 249 million cases of malaria and 608,000 fatalities in 2022. A significant obstacle in malaria control is the rise and spread of resistance to antimalarial drugs, which endangers the efficacy of current therapies and undermines worldwide endeavours to eradicate the illness. The efficacy of routinely used antimalarial medicines, such as artemisinin-based combination treatments (ACTs), is increasingly endangered by the formation and spread of multi-drug resistance (MDR). The Plasmodium falciparum multidrug resistance 1 gene (Pfmdr1) is frequently linked to multi-drug resistance. This study was conducted to detect the presence and prevalence of the Pfmdr1 gene in Plasmodium falciparum isolated from malariapositive blood samples to understand its role in anti-malaria drug resistance within Ota, Ogun State, Nigeria. Ethical approval was obtained from the Covenant Health Research Ethics Committee, Ogun state, Nigeria, before the commencement of the study. Three hundred and fifty-six blood samples were obtained from outpatients attending Covenant University Medical Centre and the General Hospital, Ado-Odo Ota, Ogun State. Microscopy was used to identify P. falciparum in all samples. The prevalence of malaria was recorded at 66%, with the age group of children ≤ 10 having the highest prevalence at 80%. DNA extraction was performed to isolate P. falciparum from 50 malaria-positive blood samples. Nested PCR was performed to identify and amplify the Pfmdr1 gene and determine its mutations. The prevalence of the Pfmdr1 was recorded at 66% among samples amplified using two-step Nested PCR. Sanger Sequencing was carried out on 10 samples to provide a detailed genetic characterization of the Pfmdr1 gene and three mutations of the Pfmdr1 gene the F74L, Y184F and S164S were identified.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Malaria, Multidrug resistance, Gene Mutations, Prevalence |
| Subjects: | Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology R Medicine > RA Public aspects of medicine |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | Patricia Nwokealisi |
| Date Deposited: | 15 Oct 2024 15:56 |
| Last Modified: | 15 Oct 2024 15:56 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/18504 |
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