Akinwumi, A.R. and Nwinyi, Obinna and Ayeni, .A. O. and Mohan, S.V. (2024) Greener Technology of Producing Polyhydroxyalkanoate using Anthracene as Carbon Source. In: ICSAEES, 2024, Lagos, Nigeria.
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Abstract
The study investigated the potential of Bacillus cereus AAR-1 (OQ999178) to simultaneously degrade anthracene, a toxic environmental pollutant, and produce polyhydroxyalkanoate (PHA), an ecofriendly and sustainable biopolymer. Using a Taguchi L16 (4*3) array for optimization, it was found that a 10% seed inoculum, grown for 8 days in a minimal salt medium containing 400 ppm of anthracene and 2 g/L of NH4Cl as carbon and nitrogen sources, respectively, maximized anthracene degradation and PHA accumulation by B. cereus AAR-1. The bacterial biomass had a colony count of 1 x 106 cfu/ml and produced 286 mg/L of biopolymer, as extracted using a hypochlorite-chloroform solvent method. Fouriertransform infrared spectroscopy (FTIR) analysis confirmed the biopolymer as PHA. This study identifies a key hydrocarbon-degrading bacterium at a municipal dumpsite, which plays a significant role in environmental biotechnology by supporting cleaner and greener technologies. This contribution aligns with the goals of SDG 12 and 14
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Uncontrolled Keywords: | Bacillus cereus, Bioplastic, Polyhydroxyalkanoate, Polycyclic aromatic hydrocarbon, Dumpsite, Chemicals and Waste management |
| Subjects: | T Technology > TN Mining engineering. Metallurgy T Technology > TP Chemical technology |
| Divisions: | Faculty of Engineering, Science and Mathematics > School of Engineering Sciences |
| Depositing User: | Patricia Nwokealisi |
| Date Deposited: | 24 Feb 2025 11:54 |
| Last Modified: | 24 Feb 2025 11:54 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/18824 |
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