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A bifunctional exoglucanase-endoglucanase fusion protein

Warren, R. Anthony J. and Gerhard, B. and Gilkes, N. R. and Owolabi, Joshua B. and Kilburn, Douglas G. and Miller Jr, R. C. (1987) A bifunctional exoglucanase-endoglucanase fusion protein. Gene, 61 (3). pp. 421-427.

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A fusion was constructed between the cex gene of Cellulomonas fimi, which encodes an exoglucanase, and the cenA gene of the same organism, which encodes an endoglucanase. The cex-cenA fusion was expressed in Escherichia coli to give a fusion protein with both exoglucanase and endoglucanase activities. The fusion protein, unlike the cex and the cenA gene products from E. coli, did not bind to microcrystalline cellulose, presumably because it lacked an intact substrate-binding region. The fusion protein was exported to the periplasm in E. coli.

Item Type: Article
Uncontrolled Keywords: Recombinant DNA; • cellulase; • exo-l,4-β-glucanase; • endo-l,4-β-glucanase; • fusion protein
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mrs Patricia Nwokealisi
Date Deposited: 16 Oct 2014 11:13
Last Modified: 16 Oct 2014 11:13

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