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Methods of Extraction and Antimicrobial Susceptibility Testing of Plant Extracts

Abah, S. E. and Egwari, L. O. (2011) Methods of Extraction and Antimicrobial Susceptibility Testing of Plant Extracts. African Jownal of Basic & Applied Sciences. pp. 205-209. ISSN 2079-2034

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Abstract

Plant extracts are widely used in pharmaceutical, food and cosmetic industry; however the effect of conventional methods of extraction described in this paper as cold and soxhlet method has not been investigated. Antimicrobial activity of Anchomanes difformis (Blume) Engl. leaf extracts have been described comparing cold and soxhlet method of extraction. The ammmt of extractable plant extract/phytochemicals, the solubility of the extracts and the susceptibility of the test organism to the extract had a correlation with the extraction methods. Cold extract had wider zones of inhibition and activity than soxhlet extract. Though there was no significant difference (P>0.05) between cold and soxhlet extracts on the entire test organisms, differences exist when compared individually. This paper revealed cold method of extraction as a better alternative to soxhlet method in antimicrobial susceptibility assay. However, the decision on the methods of extraction to be used could also be dependent on the ammmt of certain phytochemicals required and the solubility of the extracts in solvents after extraction.

Item Type: Article
Uncontrolled Keywords: Extraction methods · Plants · Antimicrobial
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mrs Patricia Nwokealisi
Date Deposited: 10 Nov 2014 12:10
Last Modified: 10 Nov 2014 12:10
URI: http://eprints.covenantuniversity.edu.ng/id/eprint/2938

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