Obembe, Olawole O.
Differential expression of cellulose synthase (CesA) gene transcripts
in potato as revealed by QRT-PCR.
Plant Physiology and Biochemistry, 47.
Two transgenic potato lines, csr2–1 and csr4–8 that contained two different antisense cellulose synthase
(CesA) genes, csr2 and csr4, respectively were crossed. The aim, amongst others, was to investigate the
possibility of generating double transformants to validate a hypothetical presence of the proteins of the
two CesA genes in the same cellulose synthase enzyme complex. SYBR-Green quantitative real-time
reverse transcription polymerase chain reaction (RT-PCR) assays were carried out on four CesA gene
transcripts (CesA1, 2, 3, and 4) in the wild type genetic background, and on the two antisense CesA gene
transcripts (CesA2 and 4) in the progeny resulting from the cross between the two transgenic potato
lines. The quantitative RT-PCR analyses revealed different expression patterns of the two CesA genes. The
CesA2 mRNA was shown to be relatively more abundant than CesA4 mRNA, regardless of the genetic
background, suggesting that the two proteins are not present in the same enzyme complex.
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