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Toxicological evaluation of precocene II isolated from Ageratum conyzoides L. (Asteraceae) in Sprague Dawley rats

Adebayo, H. A. and Zeng, Guang-Zhi and Zhang, Yu-Mei and JI, Chang-Jiu and Akindahunsi, A. A. and Tan, Ning-Hua (2010) Toxicological evaluation of precocene II isolated from Ageratum conyzoides L. (Asteraceae) in Sprague Dawley rats. African Journal of Biotechnology, 9 (20). pp. 2938-2944. ISSN 1684-5315

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Precocene II (6,7-dimethoxy-2,2-dimethyl-2-chromene) was the main constituent isolated from Ageratum conyzoides L. and reportedly possessed antifungal activity. The study investigated the isolation, purification and toxicological effects of precocene II from A. conyzoides in Sprague Dawley rats. Precocene II was isolated from the petroleum ether fraction of the plant and the structure was determined by 1H-,13C-,DEPT-NMR and MS spectral techniques. Three groups of eight rats per group were used for the study. While groups B and C were respectively administered with 25 and 50 mg/kg of precocene II in 0.25% CMC-Na for 11 days by gastric intubation, group A was administered with 0.25% CMC-Na and served as the control group. After the last treatment, animals were fasted overnight and on the 12th day, they were injected intravenously with 0.2 ml/kg body weight of phenobarbital. Animals were subsequently dissected from the abdominal region; blood was collected from the pulmonary vein into EDTA anti-coagulated and non anti-coagulated tubes. The liver, kidney and spleen tissues were extracted into separate bottles for histopathological examinations. Results from hematological study indicated that the white blood cell (WBC), red blood cell (RBC), plateletcrit (PCT) and mean corpuscular hemoglobin count (MCHC) were significantly higher across the treated groups. Biochemical result showed that serum glucose level was significantly reduced in the treated groups. No apparent damage was noticed in the liver, kidney and spleen tissues. The result therefore suggests that precocene II possesses hypoglycemic property and could alter some hematopoietic elements but was not toxic to the liver, kidney and spleen tissues.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mr Adewole Adewumi
Date Deposited: 04 Jul 2011 18:32
Last Modified: 13 Dec 2011 21:13

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