Obembe, Olawole O.
Irregular deposition of cell wall polymers resulting from defective cellulose synthase complexes.
Indian Journal of Science and Technology, 2 (3).
ISSN 0974- 6846
The crystalline cellulose microfibril is formed by
the spontaneous association of about 36 β-D-glucan
chains, which are simultaneously synthesised by a large
membrane-localised multi-enzyme cellulose synthase
complex. Antisense technology has been previously
employed separately on two of the constituent cellulose
synthase catalytic subunits (CesA) of the complex in
potato (Solanum tuberosum), namely CesA2 and CesA4,
to generate potato tuber cell walls with reduced cellulose
content. Genetic crossing of two transgenic potato lines
csr2-1 and csr4-8 was carried out to investigate the
effects of two defective CesAs in the same genetic
background, with respect to cellulose deposition in the
potato tuber cell walls. It was striking to observe, through
fluorescence microscopy with calcofluor white, a strong
fluorescence in the cell corners and less prominent and
uneven fluorescence around the cells of the csr2 tubers
as compared to others. It was also noted that these
phenotypes were not pronounced in the csr2/csr4 double
transformants as expected.
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