Obembe, Olawole O.
(2010)
Endogenous levels of reducing sugars, free amino acids and phenols during
various stages of in vitro culture of cotton (Gossypium Spp.).
International Journal of Biological and Chemical Sciences, 4 (6).
pp. 2371-2378.
ISSN 1991-8631
Abstract
Somatic embryogenesis is widely preferred as the regeneration route for in vitro studies in cotton.
However, the regeneration efficiency through this approach is low; a problem that is believed to be as a result
of the biochemical properties of the plant. The objective of this study was to investigate possible relationships
between three biochemical factors (reducing sugars, phenols, and free amino acids) and somatic
embryogenesis. In vitro cultures of the different embryogenic and non-embryogenic cultivals were established.
The levels of reducing sugars, phenols and free amino acids were determined at different developmental stages
of the cultures. Higher levels of reducing sugars and lower level of phenol were observed in embryogenic
cultivars compared to their non-embryogenic counterparts. There was a general increase in the levels of free
amino acids, which decreased with time in the highly embryogenic cultivars, whereas the levels remained high
in the poorly embryogenic and non-embryogenic cultivars. The higher content of phenols and free amino acids
may be implicated in the poor somatic embryogenic response. The data show that there are factors that may
serve as markers of somatic embryogenesis in cotton, which need to be empirically determined for any
particular cultivar chosen for genetic improvement through embryogenesis.
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