HIGH FREQUENCY MULTIPLE SHOOTS INDUCTION AND PLANT REGENERATION IN SIX ELITE INDIAN COTTON CULTIVARS

Obembe, Olawole O. (2011) HIGH FREQUENCY MULTIPLE SHOOTS INDUCTION AND PLANT REGENERATION IN SIX ELITE INDIAN COTTON CULTIVARS. Canadian Journal of Pure and Applied Sciences, Vol. 5 (1). pp. 1385-1389. ISSN 1715-9997

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Abstract

Direct multiple shoot induction and regeneration from the cotyledonary nodal explants of two Indian cultivars of upland cotton, G. hirsutum (hybrid H8 and Khandwa-2) and four cultivars of G. arboretum (BD-1, BD-6, Sarvottam and Jawahar Tapti BD) were investigated, using varying concentrations of BAP. An overall average of 5.5 shoots per explant was achieved in the study. The best multiple shoots formation (9 shoots per explant) was obtained from the two G. hirsutum cultivars cultured on 3.0 mg L-1 BAP. Shoots were harvested and elongated in the presence of 0.5 mg L-1 GA3. Root formation was achieved on hormone-free MS medium.

Item Type:	Article
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	Dr. Olawole O. Obembe
Date Deposited:	15 Jul 2011 15:56
Last Modified:	13 Dec 2011 21:13
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/437

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