Obembe, Olawole O.
HIGH FREQUENCY MULTIPLE SHOOTS INDUCTION AND PLANT
REGENERATION IN SIX ELITE INDIAN COTTON CULTIVARS.
Canadian Journal of Pure and Applied Sciences, Vol. 5 (1).
Direct multiple shoot induction and regeneration from the cotyledonary nodal explants of two Indian cultivars of upland
cotton, G. hirsutum (hybrid H8 and Khandwa-2) and four cultivars of G. arboretum (BD-1, BD-6, Sarvottam and
Jawahar Tapti BD) were investigated, using varying concentrations of BAP. An overall average of 5.5 shoots per explant
was achieved in the study. The best multiple shoots formation (9 shoots per explant) was obtained from the two G.
hirsutum cultivars cultured on 3.0 mg L-1 BAP. Shoots were harvested and elongated in the presence of 0.5 mg L-1 GA3.
Root formation was achieved on hormone-free MS medium.
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