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Equilibrium Studies of the Reaction of Turkey (Meleagris gallopavo) Haemoglobin Sulphydryl Groups with 5,5′-dithiobis(2-nitrobenzoate): Tertiary Conformational Change in Turkey Haemoglobin Induced by Inositol hexakisphosphate

Ehi-Eromosele, C. O. and Edobor-Osoh, A. and Ajanaku , C. O. and Anake, W. U. and Aladesuyi, O. and Oduselu, G. O (2014) Equilibrium Studies of the Reaction of Turkey (Meleagris gallopavo) Haemoglobin Sulphydryl Groups with 5,5′-dithiobis(2-nitrobenzoate): Tertiary Conformational Change in Turkey Haemoglobin Induced by Inositol hexakisphosphate. South Asian Journal of Experimental Biology, 4 (4). pp. 200-206. ISSN 2230-9799

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Abstract

The red blood cell of turkey contains two haemoglobin types, major and mi-nor components. In the present study, the equilibrium constant, Kequ, for the reaction of 5,5′-dithiobis(2-nitrobenzoate), DTNB, with the sulphydryl group of the major turkey aquomethaemoglobin was determined at 25°C as a function of pH. Kequ varies by about 2 to 3 orders of magnitude between pH 5.6 and 9.0 for both haemoglobin [stripped and in the presence of inositol hexakisphosphate (inositol-P6)]. Calculations from the pH dependence of Kequ showed that in the r ⇌ t tertiary conformational transition of aquomethae-moglobin, the t isomer population was 0.26 %. In the presence of inositol-P6, the t isomer population increased to 9.08 %. The results showed that while inositol-P6 increased the relative population of the t tertiary conformation by changing the relative distribution of two protein conformations, it had no effect on Kequ. The effect of Inositol-P6 on the nature and number of groups linked to the DTNB reaction was also determined.

Item Type: Article
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User: Mrs Abiola Edobor-Osoh
Date Deposited: 09 Apr 2015 14:06
Last Modified: 09 Apr 2015 14:06
URI: http://eprints.covenantuniversity.edu.ng/id/eprint/4699

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