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Sub-Cellular Correlation of Nitrite in Cassava (Manihot Esculenta Crantz) Leaves and Nitrosamine Toxicology in Wistar Rats

Efuruibe, A. A. and Akpabio, C. J. and Maduagwu, E. N. (2013) Sub-Cellular Correlation of Nitrite in Cassava (Manihot Esculenta Crantz) Leaves and Nitrosamine Toxicology in Wistar Rats. International Journal of Toxicological and Pharmacological Research, 5 (3). pp. 59-62. ISSN 0975-5160

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The aim of this study was to determine the nitrite levels in the sub-cellular fractions of cassava (Manihot esculenta crantz) leaves containing chloroplast, mitochondria and in the cytosol respectively and to their contribution in the causation of liver toxicity in rats exposed to N-nitrosamine precursors. The design of this experiment consisted of three animal groups. The first group was administered with dimethylamine hydrochloride plus sodium nitrite (DMA.HCL + NaNO2), the second group was administered with sodium nitrite (NaNO2) only and the third group (control) was given distilled water. The methods used included cell fractionation, tissue homogenization and centrifugation, spectrophotometric analysis, enzymatic determination and histopathology. Nitrite levels were estimated at 6.08 ± 0.92, 4.06 ± 1.65 and 1.29 ± 1.66μg/200g of cassava leaf tissue in chloroplasmic, mitochondrial and cytosolic sub-cellular fractions respectively. Both the NaNO2 dose regime and the combined dose of DMA.HCL and NaNO2, at P- value 0.05, caused significant increases in GGT, ALP, AST and ALT levels in serum. The histopathological study of the rat liver for DMA.HCL + NaNO2 administration showed severe portal and central venous congestion while the NaNO2 administration revealed a mild periportal cellular infiltration. This study shows that there is a correlation of nitrite in the chloroplast, mitochondria and cytosol sub-cellular fractions of cassava leaves and administration of nitrite dietary level in cassava leaves and dimethylamine hydrochloride produced acute synergistic toxicity in the liver.

Item Type: Article
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mr Adewole Adewumi
Date Deposited: 13 Apr 2015 17:14
Last Modified: 13 Apr 2015 17:14

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