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Antimicrobial effects of components of the bark extract of neem (Azadirachta indica A. J uss)

De, Nandita B. and Ifeoma, E. (2002) Antimicrobial effects of components of the bark extract of neem (Azadirachta indica A. J uss). TECHNOLOGY AND DEVELOPMENT, 8. pp. 23-28.

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Preliminary phytochemical analysis showed that the leaves and bark of A. indica possess saponins, tannins and phenolic compounds. The antimicrobial spectrum of the aqueous, methanol and acetone extracts of leaves and bark of the plant was determined by agar diffusion method. The methanol and acetone extracts of the bark at 1 Omg/ml concentration showed significant antimicrobial activities against Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Trichophyton mentagrophytes. The minimtim inhibitory concentration (MIC) values ofthe methanol extract of the bark (0.3-0.4 mg/ml) were same or lower than those of ampicillin, erythromycin and griseofulvin (0.2-0.5 mg/ml). The minimum bactericidal concentration (MBC) values (0.3-0.9 mg/ml) and the minimum fungicidal concentration 'values (MFC) (0.3-2.0 mg/ml) of the methanol extract were comparable to those of ampicillin, erythromycin (0.2-0.6 mg/ml) and griseofulvin(0.8-l.O mg/ml). Isolation of antimicrobial substances from the methanol extract of the bark was studied by thin layer chromatography (TLC) developed by using benzene and methanol mixture (4:6). Two fractions (A, B) were obtained. Fraction A (the upper band in TLC plate) showed significant activity (diameter of zone 4.0-5.0 mm at 2.0 mg/ml concentration) against C. albicans and T mentagrophytes when compared to crude extract (diameter of zone 3.0-5.0 mm at I 0 mg/ml concentration). So it may be concluded that there is a scientific basis for traditional use of extracts of bark and leaves of A. indica in skin infection and the active principles in the extracts may be purified by using TLC method.

Item Type: Article
Uncontrolled Keywords: A. indica, MIC, MBC, TLC. Plants-microbiology; skin-infections; neem (Azaddirachta A. Juss) products
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mrs Patricia Nwokealisi
Date Deposited: 23 Apr 2015 20:28
Last Modified: 23 Apr 2015 20:28

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