Comparison of Morphological., Cultural and Physiological Characteristics of an Antibiotic Product

De, N and James, H. D. (2004) Comparison of Morphological., Cultural and Physiological Characteristics of an Antibiotic Product. Nigerian Journal of Biotechnology, 15 (1). pp. 13-21. ISSN 0189-1731

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Abstract

The morphological. cultural and physiologica l characteristics of an antifungal antibiotic producing strain S kanamyceticus K2J and its non-producing mutant M6 were studied by growing the cultures in maltose sodium nitrate mineral broth (MSB), nutrient agar (NA). glucose asparagines agar (GSA). glycerol nitrate agar (GNA). potato dntrose agar (PDA), yeast peptone agar (\'PA). starch agar (SA), gelatin medium (GM), beef extract peptone nitrate broth (BPNB). yeast extract tyrosine NaCI agar (YTNA) and litmus milk (LM). The non-antibiotic producing mutant M,, shows fragmentation of mycelium into shot1er elements. while absent in the antibiotic producing strain K2.1 in maltose sodium nitrate mineral broth. The colour of the pigment produced by the two strains in NA, GSA. GNA. PDA. YPA. SA. GM and YTNA are also different. In most of the media used. antibiotic producing strain produces spores while the nonproducing strain is asporogenous. S kanannn!ticus K2J cannot utilize dulcitol as carbon source and utilizes fructose. arabinose and galactose very poorl:v <lS carbon source whereas the M6. mutant shO\\·s some amount of gro'' th in these carbon sources. M6. utilizes valine. proline. asparagines. leucine and histidine ''hereas K2J utilizes these amtno actds very well.

Item Type:	Article
Uncontrolled Keywords:	mutant. antibiotic. mycelium. asporogenou
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	Mrs Patricia Nwokealisi
Date Deposited:	24 Apr 2015 09:20
Last Modified:	24 Apr 2015 09:20
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/5107

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