Pectinolytic activity of wild-type filamentous fungi fermented on agro-wastes

Okafor, U. A. and Okochi, V. I. and Chinedu, S. N. and Ebuehi, O. A. T. and Onygeme-Okerenta, B. M. (2010) Pectinolytic activity of wild-type filamentous fungi fermented on agro-wastes. African Journal of Microbiology Research, 4 (24). pp. 2729-2734. ISSN 1996-0808

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Abstract

Five filamentous fungi (Aspergillus clavatus, Aspergillus niger, Fusarium sp., Penicillum chrysogenum and Trichoderma sp.) isolated from agrowaste samples in Lagos metropolis, Nigeria, depolymerized citrus pectin. Best pectolytic activity, as indicated by the diameter of clear, hydrolyzed zones on the medium plates containing commercial citrus pectin as sole carbon source, was obtained with A. niger, closely followed by P. chrysogenum. The two fungi also produced pectinases with different agrowastes (pineapple peel, orange peels, sawdust, sugarcane pulps and wheat bran) as the sole carbon source. The highest pectinase activity by both fungi was produce with wheat bran as the sole carbon source. Peak pectinase activity of 350.28 ± 2.82 and 478.25 ± 3.04 IU mg-1 protein was respectively obtained by submerged fermentation (SmF) at 48 h for A. niger and P.chrysogenum in media containing wheat bran as the sole carbon source. Solid-state fermentation (SSF) yielded higher levels of pectinase activity than the SmF. The strains of A. niger and P. chrysogenum have good prospect for pectinase production. Wheat bran is a good low-cost fermentation substrate for pectinase production by the investigated fungi.

Item Type:	Article
Uncontrolled Keywords:	Filamentous fungi, agro-wastes, pectinolytic activity, fermentation, pectinase production
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	Mrs Patricia Nwokealisi
Date Deposited:	25 Nov 2015 14:53
Last Modified:	25 Nov 2015 14:53
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/5667

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