Onyegeme-Okerenta, B. M. and Okochi, V. I. and Chinedu, S. N. (2013) Penicillin Production by Penicillium Chrysogenum PCL 501: Effect of UV Induced Mutation. The Internet Journal of Microbiology, 12 (1).
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Abstract
Penicillin production by Penicillium chrysogenum (PCL501) fermented on glucose, lactose and four agro-wastes (cassava shavings, corncob, sawdust, and sugarcane pulp) was monitored with HPLC. The highest amount of penicillin was obtained with sugarcane pulp. Penicillin yield from a 7-day culture of the fungus was 8.65 ± 0.05, 7.68 ± 0.03, 6.85 ± 0.05, 5.54 ± 0.01, 5.32 ± 0.05 and 2.23 ± 0.02μg/ml respectively on sugarcane pulp, glucose, cassava shavings, corncob, lactose, and sawdust. Two mutant strains, UVP1 and UVP2, were obtained by exposing the wild strain (PCL501) to ultraviolet irradiation for 20 and 25 minutes respectively. The 7-day culture of UVP1 and UVP2 on sugarcane pulp yielded 14.83 ± 0.05 and 14.97 ± 0.05 μg/ml penicillin respectively. This represented over 70% increase in penicillin production over the parent strain. There is a good prospect of producing cheaper and effective penicillin using the mutant strains of P. chrysogenum and
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | agro-wastes, mutant strains, penicillin, penicillium chrysogenum, uv irradiation |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | Mrs Patricia Nwokealisi |
| Date Deposited: | 26 Nov 2015 13:13 |
| Last Modified: | 26 Nov 2015 13:13 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/5671 |
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