Chinedu, S. N. and Okochi, V. I. and Smith, H. A. and Okafor, U. A. and Onyegeme-Okerenta, B. M. and Omidiji, O.
(2007)
Effect of carbon sources on cellulase (EC 3. 2. 1. 4)
production by Penicillium chrysogenum PCL501.
African Journal of Biochemistry Research, 1 (1).
006-010.
Abstract
The effects of glucose, crystalline cellulose and sawdust of Mitragyna cilata on the growth and cellulase
production, inferred from cellulase (EC 3. 2. 1. 4) activity, of Penicillium chrysogenum PCL501 was
determined. Glucose-containing media gave the highest mycelia weight of 1.78 mg mL-1 in 120 h of
incubation. This is about 3.5 – 4.5 times the maximum weights of 0.51 and 0.40 mg mL-1 respectively
obtained from the cultures containing cellulose and sawdust. The cultures containing crystalline
cellulose and sawdust produced extracellular protein with cellulase (EC 3. 2. 1. 4) activity whereas
glucose-containing cultures yielded very low protein and no significant cellulase activity. Maximum
protein content of 0.02, 0.13 and 0.46 mg mL-1 respectively were obtained from the cultures containing
glucose, cellulose and sawdust. Peak cellulase activity values of 100.0 and 92.2 Units L-1 respectively
were obtained for the cultures containing cellulose and sawdust. There is a correlation between the
protein released and cellulase activity of the culture filtrates. P. chrysogenum PCL501 produces
extracellular proteins with significant cellulase activity in media containing cellulose and sawdust but
not in glucose-containing medium. Sawdust is indicated as a good inducer of cellulase activity in the
organism. The waste cellulosic material can be used as low-cost carbon source for commercial
cellulase production.
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