Okafor, U. A. and Okochi, V. I. and Onyegeme-Okerenta, B. M. and Chinedu, S. N.
Xylanase production by Aspergillus niger ANL 301
using agro - wastes.
African Journal of Biotechnology, 6 (14).
Xylanase production by wild-type Aspergillus niger ANL301, newly isolated from wood-waste, was
monitored at 24 h intervals for a period 168 h in media containing different carbon sources. The carbon
sources were oat-spelt xylan (Fluka) and three agro-wastes (sawdust, sugarcane pulp and wheat bran).
Highest xylanase activity of 6.47 units/mL was obtained at 96 h in media containing wheat bran as sole
carbon source. Maximum activity value for the media containing sugarcane pulp was 0.95 units/mL
obtained also at 96 h. Sawdust and oat spelt xylan gave the peak enzyme activities of 0.65 and 0.80
units/mL respectively at 120 h. High protein yield was obtained in media containing the agro-wastes,
with wheat bran giving the highest value of 1.14 mg/mL at 96 h. The maximum specific xylanase
activities were 3.86, 3.37, 5.69, and 9.36 units/ mg protein for sawdust, sugarcane pulp, wheat bran and
oat spelt xylan, respectively. Out of the three agro-wastes used in this study, wheat bran holds greatest
promise for low cost production of the xylanase enzyme.
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