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Isolation of Cellulolytic Microfungi Involved in Wood-Waste Decomposition: Prospects for Enzymatic Hydrolysis of Cellulosic Wastes

Chinedu, S. N. and Okochi, V. I. and Smith, H. A. and Omidiji, O. (2005) Isolation of Cellulolytic Microfungi Involved in Wood-Waste Decomposition: Prospects for Enzymatic Hydrolysis of Cellulosic Wastes. International Journal of Biomedical and HealthSciences, 1 (2).

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Abstract

Wood-wastes from dump-sites at Okobaba Saw-mills on the western part of the Lagos lagoon were examined for cellulolytic microorganisms. Cellulolytic microfungi were isolated from the wastes using minimal salt agar medium containing 0.2% (w/v) crystalline cellulose, sugarcane pulp, corn cob or saw-dust as sole carbon/energy source. The colonies of cellulolytic microfungi which appeared on the plates increased in size and number as the incubation period (days) increased. Among the fungal isolates were two pathogenic Aspergilli (A flavus and A fumigatus), three different black Aspergilli (herein designated as A.niger I, A.niger II and A.niger III), Botrytis cinerea, Fusarium species and Penicillium species. Cell-free filtrates of 7 – day cultures of A.flavus, A.niger I, A.niger II, B. cinerea and P.species grown on the minimal salt broth supplemented with crystalline cellulose as sole carbon/energy source showed very significant CM–cellulase activity. P. species gave a very high value that was over 4 times the value for the closest organism, A.niger II. There is a good propect for cellulase production using the virgin strain of P. species isolated from the wood-wastes.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mr Adewole Adewumi
Date Deposited: 08 Feb 2011 14:23
Last Modified: 13 Dec 2011 21:13
URI: http://eprints.covenantuniversity.edu.ng/id/eprint/64

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