Chinedu, S. N. and Okochi, V. I. and Smith, H. A. and Omidiji, O.
Isolation of Cellulolytic Microfungi Involved in Wood-Waste
Decomposition: Prospects for Enzymatic Hydrolysis of
International Journal of Biomedical and HealthSciences, 1 (2).
Wood-wastes from dump-sites at Okobaba Saw-mills on the western part of the Lagos lagoon were examined for
cellulolytic microorganisms. Cellulolytic microfungi were isolated from the wastes using minimal salt agar medium containing
0.2% (w/v) crystalline cellulose, sugarcane pulp, corn cob or saw-dust as sole carbon/energy source. The colonies of cellulolytic
microfungi which appeared on the plates increased in size and number as the incubation period (days) increased.
Among the fungal isolates were two pathogenic Aspergilli (A flavus and A fumigatus), three different black Aspergilli (herein
designated as A.niger I, A.niger II and A.niger III), Botrytis cinerea, Fusarium species and Penicillium species. Cell-free filtrates
of 7 – day cultures of A.flavus, A.niger I, A.niger II, B. cinerea and P.species grown on the minimal salt broth supplemented with
crystalline cellulose as sole carbon/energy source showed very significant CM–cellulase activity. P. species gave a very high
value that was over 4 times the value for the closest organism, A.niger II. There is a good propect for cellulase production using
the virgin strain of P. species isolated from the wood-wastes.
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