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Cellulase Production by Wild-type Aspergillus niger, Penicillium chrysogenum and Trichoderma harzianum Using Waste Cellulosic Materials

Chinedu, S. N. and Okochi, V. I. Cellulase Production by Wild-type Aspergillus niger, Penicillium chrysogenum and Trichoderma harzianum Using Waste Cellulosic Materials. Unspecified.

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Abstract

Waste cellulosic materials (corncob, sawdust and sugarcane pulp) and crystalline cellulose induced cellulase production in wild strains of Aspergillus niger, Penicillium chrysogenum and Trichoderma harzianum isolated from a wood-waste dump in Lagos, Nigeria. Cellulose-supplemented media gave the maximum cellulase activity of 0.54, 0.67 and 0.39 units mg Protein-1 for A. niger, P. chrysogenum and T. harzianum respectively. The maximum enzyme activity for A. niger was obtained at 36 h of cultivation, while P. chrysogenum and T. harzianum gave their maximum enzyme activities at 12 and 60 h respectively. For the cellulosic wastes, highest enzyme activity was obtained with sawdust where A. niger, P. chrysogenum and T. harzianum gave the maximum enzyme activity of 0.30, 0.24 and 0.20 units mg Protein-1 respectively after 144 h of cultivation. A. niger recorded the highest enzyme activity with any of the three cellulosic materials followed by P. chrysogenum. It thus appears that the use of sawdust presents the best option for low-cost commercial production of cellulase using A. niger and P. chrysogenum as discussed herewith.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mr Adewole Adewumi
Date Deposited: 08 Feb 2011 14:32
Last Modified: 13 Dec 2011 21:13
URI: http://eprints.covenantuniversity.edu.ng/id/eprint/65

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