Biodegradation of Crude Oil, Refinery Effluent and Some Petroleum Components by Penicillium Sp. and Mortierella Sp. Isolated From Oil Contaminated Soil in Auto Mechanic Workshops

Okougbo, A.E. and De, N. and Bello, Y.M. (2016) Biodegradation of Crude Oil, Refinery Effluent and Some Petroleum Components by Penicillium Sp. and Mortierella Sp. Isolated From Oil Contaminated Soil in Auto Mechanic Workshops. In: 3rd International Conference On African Development Issues (CU-ICADI), May 9- May 11 2016, Covenant University, Ota, Nigeria.

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Abstract

Ten isolates of five different types of fungi identified as Penicillium sp. (B101F, B202F and B302F), Aspergillus sp. (B102F, B104F and B304F), Fusarium sp. (B501F), Trichoderma sp. (K602F and K561F) and Mortierella sp. (B1002F) were isolated from contaminated soil samples obtained from auto mechanic workshops in Minna, Nigeria. Mycelial extension rate measurement method was used for the isolation of the best crude oil, refinery effluent and some other petroleum hydrocarbons degraders. Two fungal strains B101F and B1002F were selected based on their ability to degrade (Lagoma light) crude oil and some other petroleum components as both carbon and nitrogen sources in mineral salt medium (MSM). The biodegradation study showed Penicillium strain B101F and Mortierella strain B1002F performed degradation at an optimum pH and temperature of 5.0 and 280C respectively. The optimum concentration of (Lagoma light) crude oil, refinery effluent and other hydrocarbons in mineral salt medium (MSM) for fungal growth was 0.5% within an incubation period of 21 days. When complex medium such as maize bran was utilized as nitrogen source with crude oil in mineral salt medium (MSM) omitting NaNO3, the optimum growth was attained on the 14th day of fermentation for both the isolates. With respect to the selected strains ability to degrade crude oil, these organisms have shown significance in reducing pollution that arise from oil spills in our environments.

Item Type:	Conference or Workshop Item (Paper)
Uncontrolled Keywords:	Biodegradation, Mycelial extension rate, Lagoma light, Mineral Salt Medium (MSM).
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	Ms Ugwunwa Esse
Date Deposited:	29 Jun 2016 13:21
Last Modified:	29 Jun 2016 13:21
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/6681

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